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BAIAP2 Antibody (OTI 1D9)

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Western Blot: BAIAP2 Antibody (1D9) [NBP2-00810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAIAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: BAIAP2 Antibody (1D9) [NBP2-00810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BAIAP2.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Human prostate tissue using anti-BAIAP2 mouse monoclonal antibody.
Flow Cytometry: BAIAP2 Antibody (1D9) [NBP2-00810] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BAIAP2 antibody, and then ...read more
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Human Kidney tissue using anti-BAIAP2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI 1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.65 mg/ml

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BAIAP2 Antibody (OTI 1D9) Summary

Immunogen
Full length human recombinant protein of human BAIAP2 (NP_006331) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BAIAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAIAP2 Antibody (OTI 1D9)

  • BAI1-associated protein 2IRS-58
  • BAI-associated protein 2
  • BAP2
  • brain-specific angiogenesis inhibitor 1-associated protein 2
  • Fas ligand-associated factor 3
  • FLAF3
  • Insulin receptor substrate p53
  • Insulin receptor substrate p53/p58
  • Insulin receptor substrate protein of 53 kDa
  • IRSP53
  • IRSp53/58
  • Protein BAP2

Background

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAIAP2 Antibody (NBP2-00810) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BAIAP2
Entrez