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B7-H4 Antibody (297219) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Rat Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB2154) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on Rat IgG2b control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Rat Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB2154) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
297219
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2154U-100UG
Alexa Fluor 405
FAB2154V-100UG
Alexa Fluor 488
FAB2154G-100UG
Alexa Fluor 532
FAB2154X
Alexa Fluor 594
FAB2154T-100UG
Alexa Fluor 647
FAB2154R-100UG
Alexa Fluor 700
FAB2154N-100UG
Alexa Fluor 750
FAB2154S-100UG
Allophycocyanin/Cy7
FAB2154APCCY7
Biotin
FAB2154B
CoraFluor 1
FAB2154CL1
DyLight 350
FAB2154D
DyLight 405
FAB2154E
DyLight 488
FAB2154K
DyLight 550
FAB2154L
DyLight 594
FAB2154M
DyLight 650
FAB2154W
DyLight 680
FAB2154Y
DyLight 755
FAB2154Z
FITC
FAB2154F
HRP
FAB2154H
Janelia Fluor 525
FAB2154JF525
Janelia Fluor 549
FAB2154I
Janelia Fluor 585
FAB2154JF585
Janelia Fluor 635
FAB2154JF635
Janelia Fluor 646
FAB2154J
Janelia Fluor 669
FAB2154JF669
PE/Atto594
FAB2154PEATT594
PE/Cy5.5
FAB2154PECY55
PE/Cy7
FAB2154PECY7
PerCP
FAB2154C
mFluor Violet 450 SE
FAB2154MFV450
mFluor Violet 500 SE
FAB2154MFV500
mFluor Violet 610 SE
FAB2154MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2154G-100UG
Carrier Free
MAB2154-SP
Carrier Free
FAB2154V-100UG
Carrier Free
FAB2154U-100UG
Carrier Free
FAB2154T-100UG
Carrier Free
FAB2154S-100UG
Carrier Free
FAB2154R-100UG
Carrier Free
FAB2154N-100UG
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B7-H4 Antibody (297219) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5
Specificity
Detects mouse B7‑H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, recombinant human (rh) B7‑H3b, rhB7‑H4, or rmPD‑L2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
VTCN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB2154 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H4 Antibody (297219) [Unconjugated]

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1

Background

B7-H4, also known as B7x and B7S1, belongs to the B7 family of costimulatory proteins and contains two extracellular Ig-like domains. B7-H4 is expressed on T cells, B cells, monocytes/macrophages, dendritic cells, and some tumor cells. B7-H4 signaling inhibits T cell activation, proliferation and maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-H4 Antibody (MAB2154)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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Bioinformatics

Gene Symbol VTCN1
Uniprot