Home » B7-H4 » B7-H4 Antibodies » B7-H4 Antibody (297219) [CoraFluor™ 1]

B7-H4 Antibody (297219) [CoraFluor™ 1]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
297219
Clonality
Monoclonal
Host
Rat
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2154U-100UG
Alexa Fluor 405
FAB2154V-100UG
Alexa Fluor 488
FAB2154G-100UG
Alexa Fluor 532
FAB2154X
Alexa Fluor 594
FAB2154T-100UG
Alexa Fluor 647
FAB2154R-100UG
Alexa Fluor 700
FAB2154N-100UG
Alexa Fluor 750
FAB2154S-100UG
Allophycocyanin/Cy7
FAB2154APCCY7
Biotin
FAB2154B
DyLight 350
FAB2154D
DyLight 405
FAB2154E
DyLight 488
FAB2154K
DyLight 550
FAB2154L
DyLight 594
FAB2154M
DyLight 650
FAB2154W
DyLight 680
FAB2154Y
DyLight 755
FAB2154Z
FITC
FAB2154F
HRP
FAB2154H
Janelia Fluor 525
FAB2154JF525
Janelia Fluor 549
FAB2154I
Janelia Fluor 646
FAB2154J
Janelia Fluor 669
FAB2154JF669
PE/Atto594
FAB2154PEATT594
PE/Cy5.5
FAB2154PECY55
PE/Cy7
FAB2154PECY7
PerCP
FAB2154C
Unconjugated
MAB2154
mFluor Violet 450 SE
FAB2154MFV450
mFluor Violet 500 SE
FAB2154MFV500
mFluor Violet 610 SE
FAB2154MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2154
Carrier Free
FAB2154G-100UG
Carrier Free
MAB2154
Carrier Free
MAB2154-SP
Carrier Free
FAB2154V-100UG
Carrier Free
FAB2154U-100UG
Carrier Free
FAB2154T-100UG
Carrier Free
FAB2154S-100UG
Carrier Free
FAB2154R-100UG
Carrier Free
FAB2154N-100UG
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B7-H4 Antibody (297219) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5
Specificity
Detects mouse B7‑H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, recombinant human (rh) B7‑H3b, rhB7‑H4, or rmPD‑L2 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
VTCN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for B7-H4 Antibody (297219) [CoraFluor™ 1]

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1

Background

B7H4 is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected, thus suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC). There are three different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VTCN1