| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2154-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB2154G-100UG | |||
| Carrier Free | MAB2154-SP | |||
| Carrier Free | FAB2154V-100UG | |||
| Carrier Free | FAB2154U-100UG | |||
| Carrier Free | FAB2154T-100UG | |||
| Carrier Free | FAB2154S-100UG | |||
| Carrier Free | FAB2154R-100UG | |||
| Carrier Free | FAB2154N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6576-SP | |||
| Carrier Free | FAB6576R-025 | |||
| Carrier Free | FAB6576R-100 | |||
| Carrier Free | FAB6576V-100UG | |||
| Carrier Free | FAB6576U-100UG | |||
| Carrier Free | FAB6576T-100UG | |||
| Carrier Free | FAB6576S-100UG | |||
| Carrier Free | FAB6576N-100UG | |||
| Carrier Free | FAB6576G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 4206-B7-01M | |||
![Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Western-Blot-NBP1-06096-img0001.jpg "Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane/cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06096-img0002.jpg)
![Flow Cytometry: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Flow-Cytometry-NBP1-06096-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30536-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.")
![Immunohistochemistry-Paraffin: B7-H4 Antibody [NBP2-30536] - Staining of human breast shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-30536-img0003.jpg)
![Western Blot: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Western blot analysis of B7-H4 on different lysates with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 ug/Lane) Lane 2: HeLa cell lysate (negative) (30 ug/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 ug/Lane) Lane 4: Mouse uterus tissue lysate (30 ug/Lane) Lane 5: Rat ovary tissue lysate (15 ug/Lane) Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32065_rabbit-b7-h4-mab-psh03-65-278202416331223.jpg "Western Blot: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Western blot analysis of B7-H4 on different lysates with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 ug/Lane) Lane 2: HeLa cell lysate (negative) (30 ug/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 ug/Lane) Lane 4: Mouse uterus tissue lysate (30 ug/Lane) Lane 5: Rat ovary tissue lysate (15 ug/Lane) Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: B7-H4 Antibody (PSH03-65) [NBP3-32065] - Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7-H4 with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H4 antibody (NBP3-32065) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32065_rabbit-b7-h4-mab-psh03-65-2920241192329.jpg)
![Western Blot: B7H4 Overexpression Lysate (Adult Normal) [NBL1-17768] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for B7H4.](http://images.novusbio.com/fullsize/B7H4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17768-img0002.jpg "Western Blot: B7H4 Overexpression Lysate (Adult Normal) [NBL1-17768] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for B7H4.")
