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B-Raf Antibody (K21-F) - V600-E Mutation

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-42702
    • Availability
      Product Discontinued

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B-Raf Antibody (K21-F) - V600-E Mutation Summary

Immunogen
Immunogen raised against B-raf.
Specificity
This antibody is specific for the detection of B-Raf V600-E mutated protein.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRAF
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM phosphate buffer, 150mM NaCl, 10mg/ml of BSA, and 0.05% of sodium azide, pH 7.4
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B-Raf Antibody (K21-F) - V600-E Mutation

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BRAF