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B-Raf Antibody (CL4003)

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Western Blot: B-Raf Antibody (CL4003) [NBP2-59034] - Analysis in human cell line MOLT-4.
Western Blot: B-Raf Antibody (CL4003) [NBP2-59034] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human cerebral cortex shows moderate positivity in neuropil and cytoplasmic staining in some neurons.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL4003
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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B-Raf Antibody (CL4003) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:PIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSS
Epitope
SAPASDSIGPQILTS
Predicted Species
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRAF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
B-Raf Recombinant Protein Antigen (NBP2-59034PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for B-Raf Antibody (CL4003)

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRAF