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ATP6V1E2 Antibody

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Western Blot: ATP6V1E2 Antibody [NBP2-15521] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000
Western Blot: ATP6V1E2 Antibody [NBP2-15521] - Western blot experiment using ATP6V1E2 Antibody at 1:90000 dilution to detect 100ng of 34kDa recombinant ATP6V1E2 protein antigen.

Product Details

Summary
Reactivity Mu, Hu, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATP6V1E2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1E2. The exact sequence is proprietary.
Predicted Species
Human (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1E2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ATP6V1E2 Antibody

  • ATP6E1V-ATPase subunit E 2
  • ATP6EL2
  • ATP6V1EL2
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD-like 2
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 2
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
  • MGC9341
  • Vacuolar proton pump subunit E 2
  • vacuolar-type proton-translocating ATPase subunit E1
  • VMA4
  • V-type proton ATPase subunit E 2

Background

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATP6V1E2
Uniprot