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ATP6V1G2 Antibody (2E11)

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Western Blot: ATP6V1G2 Antibody (2E11) [H00000534-M02] - Analysis of ATP6V1G2 expression in transfected 293T cell line by ATP6V1G2 monoclonal antibody (M02), clone 2E11.Lane 1: ATP6V1G2 transfected lysate(13.6 KDa).Lane ...read more
Western Blot: ATP6V1G2 Antibody (2E11) [H00000534-M02] - ATP6V1G2 monoclonal antibody (M02), clone 2E11. Analysis of ATP6V1G2 expression in PC-12.
Western Blot: ATP6V1G2 Antibody (2E11) [H00000534-M02] - ATP6V1G2 monoclonal antibody (M02), clone 2E11 Analysis of ATP6V1G2 expression in HepG2.
Western Blot: ATP6V1G2 Antibody (2E11) [H00000534-M02] - ATP6V1G2 monoclonal antibody (M02), clone 2E11. Analysis of ATP6V1G2 expression in Raw 264.7.
Western Blot: ATP6V1G2 Antibody (2E11) [H00000534-M02] - ATP6V1G2 monoclonal antibody (M02), clone 2E11. Analysis of ATP6V1G2 expression in NIH/3T3.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clone
2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ATP6V1G2 Antibody (2E11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ATP6V1G2 (NP_569730, 41 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QMEVEQYRREREHEFQSKQQAAMGSQGNLSAEVEQATRRQVQGMQSSQQRNRERVLAQLLGMVCDVRPQVHPNYRISA
Specificity
ATP6V1G2 - ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1G2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATP6V1G2 Antibody (2E11)

  • ATP6G2
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump)
  • ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2
  • ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
  • Em:AC004181.3
  • subunit G2
  • vacuolar ATP synthase subunit G 2
  • vacuolar proton pump G subunit 2
  • Vacuolar proton pump subunit G 2
  • V-ATPase 13 kDa subunit 2
  • V-ATPase subunit G 2
  • Vma10
  • V-type proton ATPase subunit G 2

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP6V1G2
Entrez
OMIM
Uniprot