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ATG4C Antibody (S08-8G9)

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Western Blot: ATG4C Antibody (S08-8G9) [NBP3-15003] - Western blot detection of ATG4C in K562, Hela cell lysates using ATG4C Rabbit mAb (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 53kDa.
Immunohistochemistry-Paraffin: ATG4C Antibody (S08-8G9) [NBP3-15003] - Immunohistochemistry of ATG4C in paraffin-embedded Human lung cancer tissue using ATG4C Rabbit mAb at dilution 1/50

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
S08-8G9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATG4C Antibody (S08-8G9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human ATG4C (Uniprot#: Q96DT6)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ATG4C
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for ATG4C Antibody (S08-8G9)

  • APG4 autophagy 4 homolog C (S. cerevisiae)
  • APG4 autophagy 4 homolog C
  • APG4C
  • ATG4 autophagy related 4 homolog C (S. cerevisiae)
  • AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae)
  • AUTL1
  • AUTL3APG4-C
  • AUT-like 1, cysteine endopeptidase
  • AUT-like 3 cysteine endopeptidase
  • autophagin-3
  • Autophagy-related cysteine endopeptidase 3
  • Autophagy-related protein 4 homolog C
  • cysteine protease ATG4C
  • EC 3.4.22
  • EC 3.4.22.-
  • FLJ14867

Background

ATG4C is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for ATG4C Antibody (NBP3-15003)

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Blogs on ATG4C.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

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Bioinformatics

Gene Symbol ATG4C