| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP, ICC/IF |
| Clone | 514914 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A isoform 1 Glu2-Val398 Accession # Q8WYN0 |
| Specificity | Detects human ATG4A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4B is observed. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATG4A |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Autophagy related protein 4 homolog A (ATG4A), also known as autophagin 2, is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the autophagin protein family which is involved in autophagy, a biological process in which endogenous proteins and damaged organelles are destroyed. Human ATG4A shares 100%, 94% and 93% identity with that of chimpanzee, dog and mouse.
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Secondary Antibodies |
Isotype Controls |
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ATG4A - protease that initiates ATG8 lipidation during autophagosome elongation There are 3 major autophagy pathways- microautophagy, chaperone-mediated autophagy, and macroautophagy. Macroautophagy is the pathway herein referred to as simply autophagy. Autophagy can be broken down into 4 main stages: phagophore nucleatio... Read full blog post. |
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