ATG16L1 Antibody - BSA Free

Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Alligator.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB110-60928	Applications	Species
Falvo S, Latino D, Santillo A et al. Effects of a high-fat diet on rat epididymis Journal of experimental zoology. Part A, Ecological and integrative physiology 2023-04-03 [PMID: 37009779] (WB, Rat)	WB	Rat
Bi Y, Yang G, Guo Z et al. Chronic high?salt intake induces cardiomyocyte autophagic vacuolization during left ventricular maladaptive remodeling in spontaneously hypertensive rats Experimental and Therapeutic Medicine 2023-02-16 [PMID: 36911373] (WB, Rat)	WB	Rat
He Z, Chen H, Zhong Y, Yang Q et Al. MicroRNA 223 Targeting ATG16L1 Affects Microglial Autophagy in the Kainic Acid Model of Temporal Lobe Epilepsy Front Neurol 2021-08-12 [PMID: 34381417]
Zhang Y, Xu X, Hu M et al SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy Cell Death Differ 2020-10-22 [PMID: 33087875] (Mouse) Details: Citation using the Alexa Fluor 488 version of this antibody.		Mouse
Chen F, Amgalan D, Kitsis RN et al. ATG16L1 autophagy pathway regulates BAX protein levels and programmed cell death J. Biol. Chem. 2020-08-26 [PMID: 32848017]
Hale A, Merchant M, White M Detection and analysis of autophagy in the American alligator (Alligator mississippiensis) J. Exp. Zool. B Mol. Dev. Evol. 2020-02-15 [PMID: 32061056] (WB, Alligator)	WB	Alligator
Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014-12-05 [PMID: 25485521] (WB, Mouse) Details: p62/SQSTM1 antibody used for WB on RAW264.7 cells subjected to RANKL and M-CSF mediated osteoclast differentiation as well as hypoxic stress (Fig. 1E )	WB	Mouse
Tang JY, Hsi E, Huang YC et al. Overexpression of Autophagy-Related 16-Like 1 in Patients with Oral Squamous Cell Carcinoma. Pathol. Oncol. Res. 2014-07-25 [PMID: 25060858] (IHC-P, Human) Details: ATG16L1 antibody used for IHC-P (1:250 dilution) on Human Oral Squamous Cell Carcinoma tissue sections - formalin fixation, 4um paraffin sections, heat induced antigen retrieval with 0.1 M citrate buffer pH 6.0 for 10 min, primary incubation for 30 minutes RT followed by detectionwith REAL Envision Detection System - Peroxidase/DAB. Fig 1 shows cytoplasmic staining / immunoreactivity score (IRS).	IHC-P	Human
Curtis S, Jones CJ, Garrod A et al. Identification of Autophagic Vacuoles and Regulators of Autophagy in Villous Trophoblast from Normal Term Pregnancies and in Fetal Growth Restriction J Matern Fetal Neonatal Med 2012-10-08 [PMID: 23039021] (IF/IHC, Human)	IF/IHC	Human
Tattoli I, Sorbara MT, Vuckovic D et al. Amino Acid starvation induced by invasive bacterial pathogens triggers an innate host defense program Cell Host Microbe 2012-06-14 [PMID: 22704617] (WB, Human)	WB	Human
Juarez E, Carranza C, Hernandez-Sanchez F, Leon-Contreras JC, Hernandez-Pando R, Escobedo D, Torres M, Sada E. NOD2 enhances the innate response of alveolar macrophages to Mycobacterium tuberculosis in humans. Eur J Immunol;42(4):880-9. 2012-04-01 [PMID: 22531915] (Human)		Human
Plantinga TS, Crisan TO, Oosting M. Crohn's disease-associated ATG16L1 polymorphism modulates pro-inflammatory cytokine responses selectively upon activation of NOD2. Gut. 2011-03-15 [PMID: 21406388]
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: JIANFENG CHEN	WB	Human	04/28/2014	View Summary Application Western Blot Sample Tested HCT116 whole cell lysate Species Human Lot B1

Array NB110-60928

• ATG16L1 Antibodies
• ATG16L1 ELISA Kits
• ATG16L1 Lysate
• ATG16L1 Proteins