![Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Apg3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Western-Blot-NBP2-01083-img0009.jpg "Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Apg3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3H2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATG3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ATG3/APG3 Antibody (NBP2-01083)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Apg3.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunocytochemistry-Immunofluorescence-NBP2-01083-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Apg3.")
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Human tonsil using anti-Apg3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunohistochemistry-Paraffin-NBP2-01083-img0007.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Human tonsil using anti-Apg3 mouse monoclonal antibody.")
![Flow Cytometry: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Analysis of Hela cells, using anti-Apg3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Flow-Cytometry-NBP2-01083-img0001.jpg "Flow Cytometry: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Analysis of Hela cells, using anti-Apg3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apg3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Apg3.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Western-Blot-NBP2-01083-img0002.jpg "Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apg3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Apg3.")
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Apg3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunohistochemistry-Paraffin-NBP2-01083-img0003.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Apg3 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Apg3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunohistochemistry-Paraffin-NBP2-01083-img0004.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Apg3 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Apg3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunohistochemistry-Paraffin-NBP2-01083-img0005.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Apg3 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Human Kidney tissue using anti-Apg3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Apg3-Antibody-3H2-Immunohistochemistry-Paraffin-NBP2-01083-img0006.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Human Kidney tissue using anti-Apg3 mouse monoclonal antibody.")
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