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ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free

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Western Blot: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apg3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Apg3.
Western Blot: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apg3 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Apg3.
Immunohistochemistry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of paraffin-embedded Human Kidney tissue using anti-Apg3 mouse monoclonal antibody.
Flow Cytometry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Analysis of Hela cells, using anti-Apg3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Apg3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry: ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free [NBP2-72140] - Staining of paraffin-embedded Human tonsil using anti-Apg3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72140AF350
Alexa Fluor 405
NBP2-72140AF405
Alexa Fluor 488
NBP2-72140AF488
Alexa Fluor 532
NBP2-72140AF532
Alexa Fluor 594
NBP2-72140AF594
Alexa Fluor 647
NBP2-72140AF647
Alexa Fluor 700
NBP2-72140AF700
Alexa Fluor 750
NBP2-72140AF750
Allophycocyanin
NBP2-72140APC
Allophycocyanin/Cy7
NBP2-72140APCCY7
Biotin
NBP2-72140B
CoraFluor 1
NBP2-72140CL1
DyLight 350
NBP2-72140UV
DyLight 405
NBP2-72140V
DyLight 488
NBP2-72140G
DyLight 550
NBP2-72140R
DyLight 594
NBP2-72140DL594
DyLight 650
NBP2-72140C
DyLight 680
NBP2-72140FR
DyLight 755
NBP2-72140IR
FITC
NBP2-72140F
HRP
NBP2-72140H
Janelia Fluor 525
NBP2-72140JF525
Janelia Fluor 549
NBP2-72140JF549
Janelia Fluor 585
NBP2-72140JF585
Janelia Fluor 635
NBP2-72140JF635
Janelia Fluor 646
NBP2-72140JF646
Janelia Fluor 669
NBP2-72140JF669
PE
NBP2-72140PE
PE/Atto594
NBP2-72140PEATT594
PE/Cy5.5
NBP2-72140PECY55
PE/Cy7
NBP2-72140PECY7
PerCP
NBP2-72140PCP
Unconjugated
NBP2-01083
mFluor Violet 450 SE
NBP2-72140MFV450
mFluor Violet 500 SE
NBP2-72140MFV500
mFluor Violet 610 SE
NBP2-72140MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01083
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ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ATG3/APG3 Antibody (OTI3H2) - Azide and BSA Free

  • APG3 autophagy 3-like (S. cerevisiae)
  • APG3
  • APG3L
  • APG3-LIKE
  • Apg3p
  • ATG3 autophagy related 3 homolog (S. cerevisiae)
  • ATG3
  • Autophagy-related protein 3
  • DKFZp564M1178
  • EC 6.3.2.-
  • FLJ22125,2610016C12Rik
  • hApg3
  • MGC15201
  • PC3-96
  • Protein PC3-96
  • ubiquitin-like-conjugating enzyme ATG3

Background

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATG3