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Ataxin 1 Antibody (S76-8)

Images

 
Western Blot: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, ...read more
Immunocytochemistry/ Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: ...read more
Immunocytochemistry/ Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
S76-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Ataxin 1 Antibody (S76-8) Summary

Immunogen
Synthetic peptide amino acids 164-197 (ATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPP) of mouse Ataxin-1. Rat: 100% identity (34/34 amino acids identical). Human: 88% identity (30/34 amino acids identical).
Localization
Cytoplasm , Nucleus
Specificity
Detects approx 85kDa.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ATXN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
1 ug/ml of Ataxin 1 Antibody was sufficient for detection of Ataxin-1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Ataxin 1 Antibody (S76-8)

  • ataxin 1
  • ataxin 1)
  • ataxin-1
  • ATX1spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant
  • SCA1D6S504E
  • Spinocerebellar ataxia type 1 protein

Background

Defects in Ataxin-1 are the cause of spinocerebellar ataxia type 1 (SCA1), also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 is caused by expansion of a CAG repeat in the coding region of the ataxin-1 gene. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. Ataxin-1 binds RNA in vitro and may be involved in RNA metabolism. [Uniprot]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ataxin 1 Antibody (NBP2-42186) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Ataxin 1.

ATXN2 Identified as New Genetic Risk Factor for Lou Gehrig's Disease (ALS)
Ataxin antibodies are used in the study of autosomal dominant cerebellar ataxia (ADCA) diseases. These neurodegenerative disorders are highly heterogeneous, characterized by progressive, irreversible, atrophy of the cerebellum and spinal cord.Ataxin...  Read full blog post.

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Bioinformatics

Gene Symbol ATXN1
Uniprot