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PPP2R2B Antibody

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Western Blot: PPP2R2B Antibody [NBP2-46667] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: PPP2R2B Antibody [NBP2-46667] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPP2R2B Antibody [NBP2-46667] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Western Blot: PPP2R2B Antibody [NBP2-46667] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Liver tissue, ...read more
Immunohistochemistry-Paraffin: PPP2R2B Antibody [NBP2-46667] - Staining of human cerebral cortex shows distinct cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: PPP2R2B Antibody [NBP2-46667] - Staining of human heart muscle shows no positivity in cardiomyocytes as expected.
Immunohistochemistry-Paraffin: PPP2R2B Antibody [NBP2-46667] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PPP2R2B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2R2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PPP2R2B Recombinant Protein Antigen (NBP2-46667PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PPP2R2B Antibody

  • B55BETA
  • FLJ95686
  • MGC24888
  • PP2A subunit B isoform B55-beta
  • PP2A subunit B isoform beta
  • PP2A subunit B isoform PR55-beta
  • PP2A subunit B isoform R2-beta
  • PP2A, subunit B, B-beta isoform
  • PP2AB55BETA
  • PP2ABBETA
  • PP2APR55B
  • PP2APR55BETA
  • PR2AB55BETA
  • PR2ABBETA
  • PR2APR55BETA
  • PR52B
  • PR55BETA
  • PR55-BETA
  • protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform
  • protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
  • protein phosphatase 2, regulatory subunit B, beta
  • SCA12
  • serine/threonine protein phosphatase 2A, 55 kDa regulatory subunit B, betaisoform
  • serine/threonine protein phosphatase 2A, neuronal isoform
  • serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B betaisoform
  • spinocerebellar ataxia 12

Background

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in the 5' UTR of this gene may cause a rare form of autosomal dominant spinocerebellar ataxia 12 (SCA12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPP2R2B
Entrez
Uniprot