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ARFGAP1 Antibody (OTI1F6)

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Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.
Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody, and then ...read more
Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human Kidney tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human liver tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human lymphoma tissue using anti-ARFGAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human pancreas tissue using anti-ARFGAP1 mouse monoclonal antibody.
Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - Analysis of Hela cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - Analysis of Jurkat cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.63 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72013AF350
Alexa Fluor 405
NBP2-72013AF405
Alexa Fluor 488
NBP2-72013AF488
Alexa Fluor 532
NBP2-72013AF532
Alexa Fluor 594
NBP2-72013AF594
Alexa Fluor 647
NBP2-72013AF647
Alexa Fluor 700
NBP2-72013AF700
Alexa Fluor 750
NBP2-72013AF750
Allophycocyanin
NBP2-72013APC
Allophycocyanin/Cy7
NBP2-72013APCCY7
Biotin
NBP2-72013B
CoraFluor 1
NBP2-72013CL1
DyLight 350
NBP2-72013UV
DyLight 405
NBP2-72013V
DyLight 488
NBP2-72013G
DyLight 550
NBP2-72013R
DyLight 594
NBP2-72013DL594
DyLight 650
NBP2-72013C
DyLight 680
NBP2-72013FR
DyLight 755
NBP2-72013IR
FITC
NBP2-72013F
HRP
NBP2-72013H
Janelia Fluor 525
NBP2-72013JF525
Janelia Fluor 549
NBP2-72013JF549
Janelia Fluor 646
NBP2-72013JF646
Janelia Fluor 669
NBP2-72013JF669
PE
NBP2-72013PE
PE/Atto594
NBP2-72013PEATT594
PE/Cy5.5
NBP2-72013PECY55
PE/Cy7
NBP2-72013PECY7
PerCP
NBP2-72013PCP
mFluor Violet 450 SE
NBP2-72013MFV450
mFluor Violet 500 SE
NBP2-72013MFV500
mFluor Violet 610 SE
NBP2-72013MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72013
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ARFGAP1 Antibody (OTI1F6) Summary

Immunogen
Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ARFGAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000-2000
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.63 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARFGAP1 Antibody (OTI1F6)

  • ADP-ribosylation factor 1 GTPase activating protein
  • ADP-ribosylation factor 1 GTPase-activating protein
  • ADP-ribosylation factor GTPase activating protein 1
  • ADP-ribosylation factor GTPase-activating protein 1
  • ARF GAP 1
  • ARF1 GAP
  • ARF1-directed GTPase-activating protein
  • ARF1GAP
  • ARFGAP1
  • bA261N11.3
  • FLJ10767
  • GAP protein
  • HRIHFB2281
  • MGC39924

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ARFGAP1 Antibody
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NBP2-00513
ARFGAP1 Antibody (OTI1F6)
Species: Hu, Mu, Rt, Pm
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Bioinformatics

Gene Symbol ARFGAP1
Entrez