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Aquaporin-3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining in human esophagus and skeletal muscle tissues . Corresponding AQP3 RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin-3 Antibody [NBP2-33872] - Staining of human cell line RT4 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining of human esophagus shows membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining of human kidney shows membranous positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining of human prostate shows membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Aquaporin-3 Antibody [NBP2-33872] - Staining of human skin shows membranous positivity in epidermal cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Aquaporin-3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QLMIGCHLEQPPPSNEEENVKLAHVKHKEQI
Predicted Species
Mouse (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aquaporin-3 Protein (NBP2-33872PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aquaporin-3 Antibody

  • AQP-3
  • aquaglyceroporin-3
  • aquaporin 3 (GIL blood group)
  • aquaporin 3 (Gill blood group)
  • aquaporin 3
  • aquaporin-3
  • GIL
  • Gill blood group

Background

Aquaporin 3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to themajor intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting ductcells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transportof nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that waterchannels can be functionally heterogeneous and possess water and solute permeation mechanisms. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Aquaporin-3 Antibody (NBP2-33872). (Showing 1 - 1 of 1 FAQ).

  1. I am from China and I would like to know more about your product number:NBL1-07638. We know that the theoretical MW of AQP3 protein is 31KD. But in your product specification, we can see that so many bands appear after overexpressing AQP3 with plasmid in 293 cell. Can you explain this phenomenon? Apart from theoretical strip, are all other nonspecific bands? Or is the AQP3 itself ? 
    • Yes, the theoretical size of AQP3 is 31 kDa, which would be expected under reduced conditions with a sample. We have not characterized any of the other bands in the overexpressed lysate lane. Because this is a native lysate, any modifications that may take place could cause smearing and extra bands in the lysate sample. AQP3 is extensively modified because it is a glycoprotein, glycosylation often shows up as multiple bands with smearing when run under native conditions.  This overexpression lysate is meant to only act as a positive control in WB experiments requiring a native control. Furthermore, the lane on the right side of the blot is showing detection of the lysate using an anti-Flag tag antibody, since the construct used for overexpression contains a Flag tag. 

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP3
Uniprot