Apolipoprotein A-IV/ApoA4 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Analysis in human small intestine and liver tissues. Corresponding APOA4 RNA-seq data are presented for the ...read more
Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Analysis in control (vector only transfected HEK293T lysate) and APOA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Apolipoprotein A-IV/ApoA4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Apolipoprotein A-IV/ApoA4 Protein (NBP1-86179PEP)
Publications
Read Publications using NBP1-86179.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-86179
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Applications: WB, IHC

Publications for Apolipoprotein A-IV/ApoA4 Antibody (NBP1-86179)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NBP1-86179). (Showing 1 - 1 of 1 FAQs).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOA4