Apc10 Antibody

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Immunoprecipitation:Apc10 AntibodyNBP3-30725] -Detection of human ANAPC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Apc10 Antibody Summary

Immunogen
Between 1 and 50
Assay Type
[Assay Type]
Sample Volume
[Sample Volume]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANAPC10
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate pH 7 -8.
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for Apc10 Antibody

  • anaphase promoting complex subunit 10
  • anaphase-promoting complex subunit 10
  • APC10DOC1
  • Cyclosome subunit 10
  • DKFZP564L0562

Background

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANAPC10