Apc10 Antibody

Images

 
Western Blot: Apc10 Antibody [NBP2-15423] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Apc10 Antibody [NBP2-15423] - Paraffin-embedded colon carcinoma. Apc10 antibody [N1C3] dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Apc10 Antibody Summary

Immunogen
Full length human Apc10 Recombinant protein.
Predicted Species
Mouse (99%), Rat (99%), Porcine (99%), Bovine (99%), Zebrafish (90%), Chicken (97%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANAPC10
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Apc10 Antibody

  • anaphase promoting complex subunit 10
  • anaphase-promoting complex subunit 10
  • APC10DOC1
  • Cyclosome subunit 10
  • DKFZP564L0562

Background

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANAPC10
Uniprot