Home » Angiopoietin-like Protein 1/ANGPTL1 » Angiopoietin-like Protein 1/ANGPTL1 Antibodies » Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated]
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Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated]

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Angiopoietin‑like Protein 1/ANGPTL1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2204) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to vascular endothelial cells in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Angiopoietin‑like Protein 1/ANGPTL1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2204
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Catalog# & Formulation Size Price
Carrier Free
AF2204-SP
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Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 1/ANGPTL1
Ala39-Asp491
Accession # O95841
Specificity
Detects human Angiopoietin-like 1/ANGPTL1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Angiopoietin-like 1/ANGPTL1, recombinant human Angiopoietin-like 2/ANGPTL2 and recombinant human Angiopoietin-like 6/ANGPTL6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANGPTL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF2204 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated]

  • ANG-3
  • ANG3angioarrestin
  • Angiopoietin like Protein 1
  • angiopoietin Y1
  • angiopoietin-3
  • angiopoietin-like 1
  • Angiopoietin-like Protein 1
  • angiopoietin-related protein 1
  • ANGPT3
  • ANGPTL1
  • AngY
  • AngY1
  • ARP1
  • ARP1angiopoietin 3
  • dJ595C2.2 (angiopoietin Y1)
  • dJ595C2.2
  • KIAA0351
  • UNQ162

Background

Angiopoietin-like 1 is a secreted glycoprotein structurally related to the angiopoietins. It contains an N-terminal coiled-coiled domain and a C-terminal fibrinogen-like domain. Unlike angiopoietins which are agonists and antagonists of the Tie-2 receptor tyrosine kinase, Angiopoietin-like 1 is an orphan ligand that does not bind Tie-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiopoietin-like Protein 1/ANGPTL1 Antibody (AF2204)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ANGPTL1
Uniprot