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Alpha-parvin/Actopaxin Antibody [Unconjugated]

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Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Parvin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Parvin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Parvin Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5556-SP
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Alpha-parvin/Actopaxin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human alpha -Parvin
Val46-Phe133
Accession # Q9NVD7
Specificity
Detects human alpha -Parvin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta -Parvin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PARVA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alpha-parvin/Actopaxin Antibody [Unconjugated]

  • Actopaxin
  • alphaParvin
  • alpha-Parvin
  • Calponin-like integrin-linked kinase-binding protein
  • CH-ILKBP
  • FLJ10793
  • FLJ12254
  • FLJ25106
  • FLJ46049
  • Matrix-remodeling-associated protein 2
  • matrix-remodelling associated 2
  • MXRA2
  • PARVA
  • parvin, alpha

Background

alpha -Parvin (parvus [Latin for "small"]-A/ alpha ; also actopaxin and PARV-A) is a 53 kDa member of the parvin family, alpha ‑actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372 amino acids (aa) in length. It contains two CH/calponin homology domains (aa 99‑202 and 265‑369), two NLSs (aa 19‑26 and 38‑40), and a recently identified paxillin binding sequence (aa 248‑256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21 aa substitution for aa 239‑372, and a second also showing a 46 aa substitution for aa 134‑372, respectively. Over aa 46‑133, human and mouse PARVA share 95% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PARVA
Uniprot