![Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-27820241631657.jpg "Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | PSH02-67 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human Alpha-mannosidase II aa 51-500 / 1,144. (Uniprot: Q16706) |
| Localization | Golgi apparatus membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAN2A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 131 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MAN2A1 |
![Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29820241420970.jpg "Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Flow cytometric analysis of U-87 MG cells labeling Alpha-mannosidase II. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32565, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29202416372118.jpg "Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Flow cytometric analysis of U-87 MG cells labeling Alpha-mannosidase II. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32565, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")