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MAN2B1 Antibody

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Western Blot: MAN2B1 Antibody [NBP3-41368] - Sample: Recombinant protein.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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MAN2B1 Antibody Summary

Immunogen
Immunized with recombinant Human MAN2B1 (Uniprot#: O00754; Ala883-Gly1011 (Uniprot#: O00754)
Clonality
Polyclonal
Host
Rabbit
Gene
MAN2B1
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5-20ug/mL
  • Immunohistochemistry 5-20ug/mL
  • Western Blot 0.2-2ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH7.4, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Antigen and protein A Affinity-purified

Alternate Names for MAN2B1 Antibody

  • alpha B, lysosomal
  • EC 3.2.1
  • EC 3.2.1.24
  • LAMAN
  • Lysosomal acid alpha-mannosidase
  • MAN2B1
  • MANB
  • Mannosidase alpha class 2B member 1
  • Mannosidase Alpha-B
  • mannosidase, alpha, class 2B, member 1

Background

MAN2B1 encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAN2B1