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alpha-Fetoprotein/AFP Antibody [Unconjugated]

 (6 Publications)
 (1 Review)
 
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Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified ...read more
a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours ...read more
alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) ...read more
Genetic Strategies: a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF, KO
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1369
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Catalog# & Formulation Size Price
Carrier Free
AF1369-SP
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alpha-Fetoprotein/AFP Antibody [Unconjugated] Summary

Immunogen
Human umbilical cord serum-derived alpha -Fetoprotein
Specificity
Detects human alpha -Fetoprotein in direct ELISAs and Western blots.
Source
N/A
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
AFP
Purity Statement
Antigen Affinity-purified from egg yolks
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated 5-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1369 in the following applications:
Publications
Read Publications using
AF1369 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody [Unconjugated]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

alpha ‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Fetoprotein/AFP Antibody (AF1369)(6)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: ELISA Capture, ICC.


Filter By Application
ELISA Capture
(1)
ICC
(1)
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Filter By Species
Human
(2)
N/A
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF1369 Applications Species
Jin, Y;Huang, Z;Xu, B;Chen, J; Localization of multiple DNAzymes as a branchedzyme-powered nanodevice for the immunoassay of tumor biomarkers Analytica chimica acta 2023-09-15 [PMID: 37455088] (ELISA Capture, Human, N/A) ELISA Capture Human, N/A
Marion Déjosez, Arturo Marin, Graham M. Hughes, Ariadna E. Morales, Carlos Godoy-Parejo, Jonathan L. Gray et al. Bat pluripotent stem cells reveal unusual entanglement between host and viruses Cell 2023-03-02 [PMID: 36812912]
Wei‐Ju Chen, Wei‐Kai Huang, Sarshan R. Pather, Wei‐Fang Chang, Li‐Ying Sung, Han‐Chung Wu et al. Podocalyxin‐Like Protein 1 Regulates Pluripotency through the Cholesterol Biosynthesis Pathway Advanced Science 2023-01-01 [PMID: 36373710]
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
S Klein, JL Dvornik, AR Yarrabothu, C Schaniel A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling Stem Cell Res, 2017-07-03;23(0):73-76. 2017-07-03 [PMID: 28925368] (ICC, Human) ICC Human
Hoi Pang Low, Ashutosh Tiwari, Jagadeesh Janjanam, Li Qiu, Chien-I Chang, William C. Strohsnitter et al. Screening Preeclamptic Cord Plasma for Proteins Associated with Decreased Breast Cancer Susceptibility Genomics, Proteomics & Bioinformatics 2013-12-05 [PMID: 24296084]

Review for alpha-Fetoprotein/AFP Antibody (AF1369) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1369:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin alpha-Fetoprotein/AFP AF1369
Enlarge 		5
reviewed by:
David Ivancic
 IHC-P Human 03/26/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman fetal liver
SpeciesHuman
LotISE0417081

Comments

CommentsFormalin/PFA-fixed paraffin-embedded sections
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 6.0
Sea Block as blocking agent for 30 minutes
Incubation is overnight at 4degC
AFP antibody 1/100 in Sea Block
Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed secondary
DAPI counterstain

Product General Protocols

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Video Protocols

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FAQs for alpha-Fetoprotein/AFP Antibody (AF1369) (0)

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Secondary Antibodies

 

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Recent Reviews

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Immunohistochemistry-Paraffin alpha-Fetoprotein/AFP AF1369
Enlarge
 David Ivancic
03/26/2018
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol AFP