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alpha-Fetoprotein/AFP Antibody (SPM334) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM334
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-37754AF350
Alexa Fluor 405
NBP2-37754AF405
Alexa Fluor 488
NBP2-37754AF488
Alexa Fluor 532
NBP2-37754AF532
Alexa Fluor 594
NBP2-37754AF594
Alexa Fluor 647
NBP2-37754AF647
Alexa Fluor 700
NBP2-37754AF700
Alexa Fluor 750
NBP2-37754AF750
Allophycocyanin
NBP2-37754APC
Biotin
NBP2-37754B
CoraFluor 1
NBP2-37754CL1
DyLight 405
NBP2-37754V
DyLight 488
NBP2-37754G
DyLight 550
NBP2-37754R
DyLight 594
NBP2-37754DL594
DyLight 650
NBP2-37754C
DyLight 680
NBP2-37754FR
DyLight 755
NBP2-37754IR
FITC
NBP2-37754F
HRP
NBP2-37754H
Janelia Fluor 525
NBP2-37754JF525
Janelia Fluor 549
NBP2-37754JF549
Janelia Fluor 585
NBP2-37754JF585
Janelia Fluor 635
NBP2-37754JF635
Janelia Fluor 646
NBP2-37754JF646
Janelia Fluor 669
NBP2-37754JF669
PE
NBP2-37754PE
PerCP
NBP2-37754PCP
Unconjugated
NBP2-32915-0.1mg
mFluor Violet 450 SE
NBP2-37754MFV450
mFluor Violet 500 SE
NBP2-37754MFV500
mFluor Violet 610 SE
NBP2-37754MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32915-0.1mg
Azide and BSA Free
NBP2-37754-0.1mg
IHC-Prediluted
NBP2-45010
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alpha-Fetoprotein/AFP Antibody (SPM334) [DyLight 350] Summary

Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771)
Localization
Cytoplasmic
Marker
Hepatocellular/Germ Cell Tumor Marker
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Bovine, Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for alpha-Fetoprotein/AFP Antibody (SPM334) [DyLight 350]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AFP