![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (SPM334) [NBP2-32915] - Formalin-paraffin human fetal liver stained with AFP MAb (SPM334).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-SPM334-Immunohistochemistry-Paraffin-NBP2-32915-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (SPM334) [NBP2-32915] - Formalin-paraffin human fetal liver stained with AFP MAb (SPM334).")
| Reactivity | Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)Species Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | SPM334 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-37754) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771) |
| Localization | Cytoplasmic |
| Marker | Hepatocellular/Germ Cell Tumor Marker |
| Specificity | It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AFP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-32915 | Applications | Species |
|---|---|---|
| Ricardo-Gonzalez RR, Kotas ME, O'Leary CE et al. Innate type 2 immunity controls hair follicle commensalism by Demodex mites Immunity 2022-08-26 [PMID: 36044899] |
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Fetoprotein/AFP Antibody (NBP2-32915)Find related products by research area.
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![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (SPM334) [NBP2-32915] - Formalin-paraffin human fetal liver stained with AFP MAb (SPM334).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-SPM334-Immunohistochemistry-Paraffin-NBP2-32915-img0005.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (SPM334) [NBP2-32915] - Formalin-paraffin human fetal liver stained with AFP MAb (SPM334).")
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