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Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488]

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Product Image: Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488] [NB110-3638AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.
Product Image: Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488] [NB110-3638AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC
Clone
8B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-3638AF350
Alexa Fluor 405
NB110-3638AF405
Alexa Fluor 532
NB110-3638AF532
Alexa Fluor 594
NB110-3638AF594
Alexa Fluor 647
NB110-3638AF647
Alexa Fluor 700
NB110-3638AF700
Alexa Fluor 750
NB110-3638AF750
Allophycocyanin
NB110-3638APC
Allophycocyanin/Cy7
NB110-3638APCCY7
Biotin
NB110-3638B
CoraFluor 1
NB110-3638CL1
DyLight 350
NB110-3638UV
DyLight 405
NB110-3638V
DyLight 488
NB110-3638G
DyLight 550
NB110-3638R
DyLight 594
NB110-3638DL594
DyLight 650
NB110-3638C
DyLight 680
NB110-3638FR
DyLight 755
NB110-3638IR
FITC
NB110-3638F
HRP
NB110-3638H
Janelia Fluor 525
NB110-3638JF525
Janelia Fluor 549
NB110-3638JF549
Janelia Fluor 646
NB110-3638JF646
Janelia Fluor 669
NB110-3638JF669
PE
NB110-3638PE
PE/Atto594
NB110-3638PEATT594
PE/Cy5.5
NB110-3638PECY55
PE/Cy7
NB110-3638PECY7
PerCP
NB110-3638PCP
Unconjugated
NB110-3638
mFluor Violet 450 SE
NB110-3638MFV450
mFluor Violet 500 SE
NB110-3638MFV500
mFluor Violet 610 SE
NB110-3638MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB110-3638
BSA Free
NB110-3638
BSA Free
NB110-3638SS
Azide and BSA Free
NBP2-80568
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Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488] Summary

Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Localization
Cell membrane; Lipid-anchor > GPI-anchor
Specificity
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ALPP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Radioimmunodiffusion
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488]

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Background

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Alkaline
Phosphatase/ALPP Anti ...
NB110-3638AF488
Alkaline Phosphatase/ALPP Antibody (8B6) [Alexa Fluor® 488]
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, Func, ICC/IF, IHC

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Product General Protocols

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FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638AF488). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

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Bioinformatics

Gene Symbol ALPP