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ALG5 Antibody - Azide and BSA Free

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Western Blot: ALG5 Antibody [NBP2-92371] - Analysis of extracts of various cell lines, using ALG5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: ALG5 Antibody [NBP2-92371] - Analysis of L929 cells using ALG5 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ALG5 Antibody [NBP2-92371] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ALG5 Rabbit pAb (NBP2-92371) at dilution of 1:100 (40x lens). Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: ALG5 Antibody [NBP2-92371] - Immunohistochemistry of paraffin-embedded Mouse pancreas using ALG5 Rabbit pAb (NBP2-92371) at dilution of 1:100 (40x lens). Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: ALG5 Antibody [NBP2-92371] - Immunohistochemistry of paraffin-embedded Rat ovary using ALG5 Rabbit pAb (NBP2-92371) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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ALG5 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 34-190 of human ALG5 (NP_037470.1). MPALHRHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDLQPWPNQM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALG5
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for ALG5 Antibody - Azide and BSA Free

  • Alg5, S. cerevisiae, homolog of
  • asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphatebeta-glucosyltransferase)
  • asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphatebeta-glucosyltransferase)
  • asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferasehomolog (S. cerevisiae)
  • Asparagine-linked glycosylation protein 5 homolog
  • bA421P11.2
  • dolichyl phosphate glucosyltransferase
  • dolichyl-phosphate beta-glucosyltransferase
  • dolP-glucosyltransferase
  • EC 2.4.1
  • EC 2.4.1.117
  • RP11-421P11.2

Background

ALG5, also known as Dolichyl-phosphate beta-glucosyltransferase, has a 37 kDa and a 34 kDa isoform, and is involved in the process of substrate recognition. Disease research is currently being conducted on ALG5 and its relation to the congenital disorder of glycosylation. The protein has been linked to several protein metabolism, biosynthesis, and modification pathways, where it interacts with ACER3, DDOST, PIGK, RPN1, and ALG12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional ALG5 Products

Array NBP2-92371

Research Areas for ALG5 Antibody (NBP2-92371)

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Bioinformatics

Gene Symbol ALG5