![Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Western-Blot-NBP1-80835-img0019.jpg "Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFD |
| Predicted Species | Mouse (98%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RPN2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-80835 | Applications | Species |
|---|---|---|
| Lyu J, Imachi H, Fukunaga K et al. Exendin-4 Increases Scavenger Receptor Class BI Expression via Activation of AMPK/FoxO1 in Human Vascular Endothelial Cells Current issues in molecular biology 2022-11-03 [PMID: 36354682] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80835-img0007.jpg "Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0018.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0013.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0014.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0015.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0016.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining in human epididymis and skeletal muscle tissues using NBP1-80835 antibody. Corresponding RPN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ribophorin-II-Antibody-Immunohistochemistry-Paraffin-NBP1-80835-img0017.jpg "Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining in human epididymis and skeletal muscle tissues using NBP1-80835 antibody. Corresponding RPN2 RNA-seq data are presented for the same tissues.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")