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DPM1 Antibody

Images

 
Western Blot: DPM1 Antibody [NBP1-05034] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DPM1 Antibody [NBP1-05034] - (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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DPM1 Antibody Summary

Immunogen
Peptide with sequence RELEVRSPRQNKYS-C corresponding to N-Terminus according to NP_003850.1.
Epitope
N Terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPM1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
Western blot: Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.6kDa according to NP_003850.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil.).
Control
DPM1 Overexpression Lysate
Publications
Read Publication using NBP1-05034.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DPM1 Antibody

  • CDGIEEC 2.4.1.83
  • dolichol monophosphate mannose synthase
  • dolichol-phosphate mannosyltransferase
  • Dolichyl-phosphate beta-D-mannosyltransferase
  • dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
  • DPM synthase
  • Mannose-P-dolichol synthase
  • MPD synthase
  • MPDSDolichol-phosphate mannose synthase

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DPM1 Antibody (NBP1-05034)(1)

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Bioinformatics

Gene Symbol DPM1
Entrez
Uniprot