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Emerin Antibody

Images

 
Genetic Strategies: Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.
Western Blot: Emerin Antibody [NBP1-87692] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human heart muscle shows strong positivity in nuclear membrane in cardiomyocytes.
Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells and smooth muscle cells with additional weak cytoplasmic ...read more
Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692]- Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts and leydig cells with weak cytoplasmic positivity.

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Emerin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EMD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF IFixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Emerin Protein (NBP1-87692PEP)
Publications
Read Publications using
NBP1-87692 in the following applications:

  • WB
    2 publications

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 27548707).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Emerin Antibody

  • emerin
  • Emery-Dreifuss muscular dystrophy
  • LEM domain containing 5
  • STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Emerin Antibody (NBP1-87692)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EMD
Entrez
Uniprot