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ALDH1L1 Antibody (OTI3D2)

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Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1.
Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human prostate tissue using anti-ALDH1L1 mouse monoclonal antibody.
Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human Kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human liver tissue using anti-ALDH1L1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.88 mg/ml

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ALDH1L1 Antibody (OTI3D2) Summary

Immunogen
Full length human recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cell.
Marker
Astrocyte Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALDH1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
98.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALDH1L1 Antibody (OTI3D2)

  • 10-formyltetrahydrofolate dehydrogenase
  • aldehyde dehydrogenase 1 family, member L1
  • aldehyde dehydrogenase family 1 member L1
  • Cytosolic 10-formyltetrahydrofolate dehydrogenase
  • DKFZp781N0997
  • EC 1.5.1.6,10-FTHFDH
  • FDH
  • formyltetrahydrofolate dehydrogenase
  • FTHFD10-fTHF

Background

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH1L1 Antibody (NBP2-03143). (Showing 1 - 1 of 1 FAQ).

  1. The Ab required is: monoclonal ALDH1L1 Ab (2A6) (NBP2-03159). ALDH1L1 has three domains: N domain , Intermediate domain and C terminal domain. Your company produces a polyclonal Ab against the domain C of ALDH1L1. Is the monoclonal ALDH1L1 Ab (2A6) (NBP2-03159) specific for the domain C? In other words, will this Ab bind the domain C? And monoclonal ALDH1L1 Ab (3D2) (NBP2-03143)?
    • The two other antibodies that you have been looking for a region of binding for NBP2-03159 & NBP2-03143, will not be known as they are raised against full length recombinant proteins. We do not epitope map these antibodies so we are unsure along the protein sequence where exactly they are binding.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALDH1L1
Entrez