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AKAP12 Antibody

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Orthogonal Strategies: Western Blot: AKAP12 Antibody [NBP1-89168] - Analysis in human cell lines U-251MG and MCF-7 using anti-AKAP12 antibody. Corresponding AKAP12 RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP1-89168] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining in human testis and skeletal muscle tissues using NBP1-89168 antibody. Corresponding AKAP12 RNA-seq data are presented ...read more
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium shows moderate positivity in cells in endometrial stroma.
Independent Antibodies: Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium, kidney, skeletal muscle and testis using Anti-AKAP12 antibody NBP1-89168 (A) shows similar ...read more
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human kidney shows strong positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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AKAP12 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDSETDGST
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation permeabilization: PFA/Triton X-100
Control Peptide
AKAP12 Recombinant Protein Antigen (NBP1-89168PEP)
Publications
Read Publication using
NBP1-89168 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AKAP12 Antibody

  • A kinase (PRKA) anchor protein (gravin) 12
  • A kinase (PRKA) anchor protein 12
  • AKAP 250
  • AKAP250250kDa
  • DKFZp686M0430
  • DKFZp686O0331
  • FLJ20945
  • FLJ97621
  • Gravin
  • kinase scaffold protein gravin
  • myasthenia gravis autoantigen gravin
  • Myasthenia gravis autoantigen

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP12 Antibody (NBP1-89168)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKAP12
Entrez
Uniprot