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![Western Blot: AKAP12 Antibody [NBP1-89168] - Analysis in human cell lines U-251MG and MCF-7 using anti-AKAP12 antibody. Corresponding AKAP12 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Western-Blot-NBP1-89168-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: AKAP12 Antibody [NBP1-89168] - Analysis in human cell lines U-251MG and MCF-7 using anti-AKAP12 antibody. Corresponding AKAP12 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP1-89168] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89168-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining in human testis and skeletal muscle tissues using NBP1-89168 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0023.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium shows moderate positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium, kidney, skeletal muscle and testis using Anti-AKAP12 antibody NBP1-89168 (A) shows similar protein distribution across tissues to independent antibody NBP2-38786 (B).](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0019.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human kidney shows strong positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0020.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0021.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-89168-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP2-38786] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38786-img0006.jpg "Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP2-38786] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining in human testis and skeletal muscle tissues using NBP2-38786 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0015.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human endometrium shows moderate positivity in stromal cells.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human endometrium, kidney, skeletal muscle and testis using Anti-AKAP12 antibody NBP2-38786 (A) shows similar protein distribution across tissues to independent antibody NBP1-89168 (B).](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0011.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human kidney shows strong positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0012.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0013.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP2-38786-img0014.jpg)
![Western Blot: AKAP12 Antibody [NBP1-31482] - Sample (30ug whole cell lysate) A:HeLa S3 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Western-Blot-NBP1-31482-img0006.jpg "Western Blot: AKAP12 Antibody [NBP1-31482] - Sample (30ug whole cell lysate) A:HeLa S3 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP1-31482] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31482-img0004.jpg)
![Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-31482] - Paraffin-embedded serous OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-31482-img0005.jpg)
![Western Blot: AKAP12 Antibody [NB100-68211] - Detection of Human AKAP12/Gravin on HeLa whole cell lysate using NB100-68211. AKAP12/Gravin was also immunoprecipitated by rabbit anti-AKAP12/Gravin antibody NB100-68212.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Western-Blot-NB100-68211-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AKAP12 Antibody [NB100-68211] - Detection of Human AKAP12/Gravin on HeLa whole cell lysate using NB100-68211. AKAP12/Gravin was also immunoprecipitated by rabbit anti-AKAP12/Gravin antibody NB100-68212.")
![Sandwich ELISA: AKAP12 Antibody (1C5) [H00009590-M01] - Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKAP12-Antibody-1C5-Sandwich-ELISA-H00009590-M01-img0002.jpg "Sandwich ELISA: AKAP12 Antibody (1C5) [H00009590-M01] - Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.")
![Western Blot: AKAP12 Antibody [NBP2-66604] - Western blotting of total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The protein was transferred to a PVDF membrane, blocked with 1X TBS, 0.1% Tween 20/ 5% NFDM, probed with 5 ug/mL of pre-immune serum 5 ug of AKAP12 antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP at 1:10000, and detected with luminol.](http://images.novusbio.com/fullsize/AKAP12-Antibody-Western-Blot-NBP2-66604-img0001.jpg "Western Blot: AKAP12 Antibody [NBP2-66604] - Western blotting of total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The protein was transferred to a PVDF membrane, blocked with 1X TBS, 0.1% Tween 20/ 5% NFDM, probed with 5 ug/mL of pre-immune serum 5 ug of AKAP12 antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP at 1:10000, and detected with luminol.")
![ELISA: Human AKAP12 ELISA Kit (Colorimetric) [NBP2-68183] - Samples were spiked with high concentrations of Human AKAP12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68183_human-akap12-elisa-kit-colorimetric-132202416221056.jpg "ELISA: Human AKAP12 ELISA Kit (Colorimetric) [NBP2-68183] - Samples were spiked with high concentrations of Human AKAP12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human AKAP12 ELISA Kit (Colorimetric) [NBP2-68183] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68183_human-akap12-elisa-kit-colorimetric-432024859776.jpg)
![Western Blot: AKAP12 Overexpression Lysate (Adult Normal) [NBL1-07426] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKAP12.](http://images.novusbio.com/fullsize/AKAP12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07426-img0002.jpg "Western Blot: AKAP12 Overexpression Lysate (Adult Normal) [NBL1-07426] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKAP12.")
![Western Blot: AKAP12 Overexpression Lysate (Adult Normal) [NBP2-07793] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKAP12.](http://images.novusbio.com/fullsize/AKAP12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07793-img0001.jpg "Western Blot: AKAP12 Overexpression Lysate (Adult Normal) [NBP2-07793] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKAP12.")
![Sandwich ELISA: AKAP12 Antibody (3C3) [H00009590-M02] - Detection limit for recombinant GST tagged AKAP12 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKAP12-Antibody-3C3-Sandwich-ELISA-H00009590-M02-img0002.jpg "Sandwich ELISA: AKAP12 Antibody (3C3) [H00009590-M02] - Detection limit for recombinant GST tagged AKAP12 is approximately 3ng/ml as a capture antibody.")