Human AKAP12 ELISA Kit (Colorimetric)

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ELISA: Human AKAP12 ELISA Kit (Colorimetric) [NBP2-68183] - Samples were spiked with high concentrations of Human AKAP12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human AKAP12 ELISA Kit (Colorimetric) [NBP2-68183] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

Human AKAP12 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human AKAP12 in samples. No significant cross-reactivity or interference between Human AKAP12 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.95%
Intra-Assay
CV% < 4.92%
Spike Recovery
86-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AKAP12

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human AKAP12 ELISA Kit (Colorimetric)

  • A kinase (PRKA) anchor protein (gravin) 12
  • A kinase (PRKA) anchor protein 12
  • AKAP 250
  • AKAP250250kDa
  • DKFZp686M0430
  • DKFZp686O0331
  • FLJ20945
  • FLJ97621
  • Gravin
  • kinase scaffold protein gravin
  • myasthenia gravis autoantigen gravin
  • Myasthenia gravis autoantigen

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AKAP12