Aggrecan Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Aggrecan, also known as aggrecan 1, chondroitin sulfate proteoglycan, and large aggregating proteoglycan, is encoded by the AGC1 gene with gene aliases of SEDK; CSPG1; MSK16; CSPGCP. As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases (2). The deduced amino acid sequence of human aggrecan core protein consists of 2415 resides and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2, and G3 (3). Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250 kDa core protein, aggrecan monomers exist as a 1,000-2,000 kDa molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10-100 aggrecan monomers on a hyaluronan backbone (2).

Aggrecan can be cleaved by MMPs and ADAMTSs at the Asn360-Phe361 and Glu392-Ala393 bond in the IGD (residues are numbered based on Accession # NP_037359), respectively (2). Inhibition of ADATMS4 and ADAMTS5 cleavage prevents aggrecan degradation in osteoarthritic cartilage, while mice with aggrecan resistant to MMP cleavage do not accumulate aggrean and develop normally (2, 4). Consisting of the G1, IGD and G2 domains, rhAggrecan can be used as a protein substrate for MMPs and ADAMTSs and in binding assays involving hyaluronan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using AF1220	Applications	Species
Gomez-Sequeda, N;Mendivil-Perez, M;Jimenez-Del-Rio, M;Lopera, F;Velez-Pardo, C; Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology Scientific reports 2023-08-08 [PMID: 37553376] (ICC, Human)	ICC	Human
Chen, MF;Hu, CC;Hsu, YH;Chiu, YT;Chen, KL;Ueng, SWN;Chang, Y; Characterization and Advancement of an Evaluation Method for the Treatment of Spontaneous Osteoarthritis in STR/ort Mice: GRGDS Peptides as a Potential Treatment for Osteoarthritis Biomedicines 2023-04-06 [PMID: 37189729] (IHC, Mouse)	IHC	Mouse
Wu, JY;Yeager, K;Tavakol, DN;Morsink, M;Wang, B;Soni, RK;Hung, CT;Vunjak-Novakovic, G; Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-? signaling Cell reports 2023-05-12 [PMID: 37178118] (IHC, Human)	IHC	Human
S Yamazaki, R Hirayama, Y Ikeda, S Iseki, T Yoda, MA Ikeda Hyaluronic acid hydrogels support to generate integrated bone formation through endochondral ossification in vivo using mesenchymal stem cells PLoS ONE, 2023-02-02;18(2):e0281345. 2023-02-02 [PMID: 36730328] (Western Blot, Human)	Western Blot	Human
Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 7/15/2022 [PMID: 35754733]
Makoto Kondo, Sumako Kameishi, Kyungsook Kim, Nicolas F. Metzler, Travis G. Maak, Douglas T. Hutchinson et al. Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment npj Regenerative Medicine 10/15/2021 [PMID: 34654830]
A Mik?osz, B ?ukaszuk, E Supruniuk, K Grubczak, M Moniuszko, B Choroma?sk, P My?liwiec, A Chabowski Does TBC1D4 (AS160) or TBC1D1 Deficiency Affect the Expression of Fatty Acid Handling Proteins in the Adipocytes Differentiated from Human Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Obtained from Subcutaneous and Visceral Fat Depots? Cells, 2021-06-16;10(6):. 2021-06-16 [PMID: 34208471] (IHC, Human)	IHC	Human
C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration Cell Res., 2020-07-31;0(0):. 2020-07-31 [PMID: 32737416] (ICC, Human)	ICC	Human
AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)	Western Blot	Mouse
R Lopez Pere, J Brauer, A Rühle, T Trinh, S Sisombath, P Wuchter, AL Grosu, J Debus, R Saffrich, PE Huber, NH Nicolay Human mesenchymal stem cells are resistant to UV-B irradiation Sci Rep, 2019-12-27;9(1):20000. 2019-12-27 [PMID: 31882818] (IHC-Fr, Human)	IHC-Fr	Human
S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (ICC, Human)	ICC	Human
Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis European Respiratory Journal 3/1/2019 [PMID: 30578393]
W Srisuthtay, D Pruksakorn, P Kongtawele, P Pothacharo Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes BMC Complement Altern Med, 2017-05-31;17(1):286. 2017-05-31 [PMID: 28569157] (Western Blot, Human)	Western Blot	Human
K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi Identification of multipotent stem cells in human brain tissue following stroke Stem Cells Dev, 2017-04-19;0(0):. 2017-04-19 [PMID: 28323540] (ICC, Human)	ICC	Human
Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC. Sci Rep, 2016-07-06;6(0):29160. 2016-07-06 [PMID: 27381039] (ICC, IHC, Human)	ICC, IHC	Human
Marfia G, Navone S, Di Vito C, Tabano S, Giammattei L, Di Cristofori A, Gualtierotti R, Tremolada C, Zavanone M, Caroli M, Torchia F, Miozzo M, Rampini P, Riboni L, Campanella R Gene expression profile analysis of human mesenchymal stem cells from herniated and degenerated intervertebral discs reveals different expression of osteopontin. Stem Cells Dev, 2014-10-29;24(3):320-8. 2014-10-29 [PMID: 25203751] (ICC, Human)	ICC	Human
Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies. PLoS ONE, 2013-01-29;8(1):e55319. 2013-01-29 [PMID: 23383153] (IHC-Fr, Human)	IHC-Fr	Human
Asumda FZ, Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 2011-10-12;12(0):44. 2011-10-12 [PMID: 21992089] (ICC, Rat)	ICC	Rat
Tautzenberger A, Lorenz S, Kreja L, Zeller A, Musyanovych A, Schrezenmeier H, Landfester K, Mailander V, Ignatius A Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation. Biomaterials, 2009-12-11;31(8):2064-71. 2009-12-11 [PMID: 20004969] (ICC, Human)	ICC	Human
Show All 19 Publications. Collapse Publications.

Array AF1220

• Aggrecan Antibodies
• Aggrecan ELISA Kits
• Aggrecan Proteins
• Aggrecan ELISA
• Aggrecan Proteins and Enzyme