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AG-3/AGR3 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: AG-3/AGR3 Antibody [NBP2-47555] - Staining in human fallopian tube and liver tissues using anti-AGR3 antibody. Corresponding AGR3 RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: AG-3/AGR3 Antibody [NBP2-47555] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AG-3/AGR3 Antibody [NBP2-47555] - Staining of human fallopian tube shows high expression.
Immunohistochemistry-Paraffin: AG-3/AGR3 Antibody [NBP2-47555] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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AG-3/AGR3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: WGDDITWVQTYEEGLFYAQKSKKPLMV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
AG-3/AGR3 Protein (NBP2-47555PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AG-3/AGR3 Antibody

  • AG3
  • AG-3
  • AGR3
  • anterior gradient homolog 3 (Xenopus laevis)
  • BCMP11
  • BCMP11AG3
  • Breast cancer membrane protein 11
  • hAG-3member 18
  • PDIA18

Background

AGR3 is a gene that encodes Anterior Gradient Homolog 3, a protein associated with breast cancer. Also known as Breat Cancer Membrane Protein 11, AG3 has a positive correlation with the expression of estrogen receptor. Its expression is negatively correlated with the expression of the EGF receptor. Because it is expressed in nearly three-quarters of breast cancer cells, it has high potential as a target for breast cancer therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AGR3