![Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Western-Blot-NBP2-45269-img0005.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 3B11NB |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant human FOXA1 protein (between amino acids 350-500) [Uniprot: P55317]. |
| Predicted Species | Mouse (98%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXA1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for HNF-3 alpha/FoxA1 Antibody (NBP2-45269)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Immunocytochemistry-Immunofluorescence-NBP2-45269-img0004.jpg "Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively")
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human prostate.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Immunohistochemistry-Paraffin-NBP2-45269-img0003.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human prostate.")
![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Flow-Cytometry-NBP2-45269-img0012.jpg "Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).")
![Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Western-Blot-NBP2-45269-img0001.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly.")
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human breast adenocarcinoma.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Immunohistochemistry-Paraffin-NBP2-45269-img0002.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human breast adenocarcinoma.")
![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-3B11NB-Flow-Cytometry-NBP2-45269-img0011.jpg "Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).")
![Western Blot: Mouse Monoclonal HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - 40 ug protein from OUMS29 cell lysates were loaded into gels for western blot experiment. The PVDF membranes were probed with this antibody at 1ug/ml concentration. Bands for HNF-3 alpha/FoxA1 appeared around 53kDa. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-45269_mouse-monoclonal-hnf-3-alpha-foxa1-antibody-3b11nb-western-blot-2122024111512..jpg "Western Blot: Mouse Monoclonal HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - 40 ug protein from OUMS29 cell lysates were loaded into gels for western blot experiment. The PVDF membranes were probed with this antibody at 1ug/ml concentration. Bands for HNF-3 alpha/FoxA1 appeared around 53kDa. Image from a verified customer review.")
![Chromatin Immunoprecipitation (ChIP) HNF-3 beta/FoxA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/HNF-3_beta_AF2400_Chromatin_Immunoprecipitation_11227.jpg)
![Immunocytochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/HNF-3_beta_AF2400_Immunocytochemistry_6484.jpg)
![Immunohistochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/HNF3_beta_AF2400_Immunohistochemistry_23167.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Immunohistochemistry Uteroglobin/SCGB1A1 Antibody (394324) [Unconjugated]](https://images.novusbio.com/images2/Uteroglobin_MAB4218_Immunohistochemistry_7019.jpg)
![Immunohistochemistry Uteroglobin/SCGB1A1 Antibody (394324) [Unconjugated]](https://images.novusbio.com/images2/Uteroglobin_MAB4218_Immunohistochemistry_9991.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Western_Blot_5199.jpg)
![Immunocytochemistry GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Immunocytochemistry_6486.jpg)
![Simple Western GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA3_AF2605_Simple_Western_16988.jpg)
