![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Western-Blot-NBP1-05936-img0011.jpg "Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide made to an N-terminal portion of mouse AGR2 (within residues 1-50). [Swiss-Prot# O88312] |
| Localization | Secreted |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AGR2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This AGR2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band can be seen at ~19.9 kDa. In ICC/IF cytoplasmic staining is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-05936 | Applications | Species |
|---|---|---|
| Maurel M, Obacz J, Avril T et al. Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation EMBO Mol Med 2019-04-30 [PMID: 31040128] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for AG-2/AGR2 Antibody (NBP1-05936)Find related products by research area.
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| Gene Symbol | AGR2 |
![Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05936-img0012.jpg "Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Representative immunohistological analysis of CD163 (scavenger receptor present in macrophages), AGR2, and TMED2 in non-inflamed colonic biopsies from healthy controls and patients with active or quiescent CD. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0014.jpg "Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Representative immunohistological analysis of CD163 (scavenger receptor present in macrophages), AGR2, and TMED2 in non-inflamed colonic biopsies from healthy controls and patients with active or quiescent CD. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.")
![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Western blot analysis (left panel) and quantification (right panel) of AGR2 intracellular expression upon TMED2 overexpression. Data are representative of four independent experiments. Tubulin (TUB) was used as a loading control. The graph represents average signal normalized on reporter protein expression +/- SD. The Mann-Whitney statistical test was used. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31040128/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2 Antibody - BSA Free-Western Blot-NBP1-05936-img0015.jpg "Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Western blot analysis (left panel) and quantification (right panel) of AGR2 intracellular expression upon TMED2 overexpression. Data are representative of four independent experiments. Tubulin (TUB) was used as a loading control. The graph represents average signal normalized on reporter protein expression +/- SD. The Mann-Whitney statistical test was used. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31040128/) licensed under a CC-BY license.")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse prostate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0009.jpg "Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse prostate.")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse kidney.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0010.jpg "Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse kidney.")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - B. Analysis of AGR2 expression in the proximal colon of WT and TMED2 hypomorph mice. C.Semi-quantitative analysis of AGR2 expression in the proximal colon of WT (blank bars) and TMED2 hypomorph mice (black bars) (n = 6). The graph represents average IHC signal +/- SD. For statistical analyses, the Mann-Whitney test was used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0013.jpg "Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - B. Analysis of AGR2 expression in the proximal colon of WT and TMED2 hypomorph mice. C.Semi-quantitative analysis of AGR2 expression in the proximal colon of WT (blank bars) and TMED2 hypomorph mice (black bars) (n = 6). The graph represents average IHC signal +/- SD. For statistical analyses, the Mann-Whitney test was used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")