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ACE-2 Antibody [Unconjugated]

 (19 Publications)
 (1 Review)
 
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Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat ...read more
HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by ...read more
ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at ...read more
ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE‑2 Antigen ...read more
Recombinant Mouse ACE‑2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3437-SP
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ACE-2 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740
Accession # Q8R0I0
Specificity
Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACE2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3437 in the following applications:
Publications
Read Publications using
AF3437 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE-2 Antibody (AF3437)(19)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


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IHC
(6)
IHC-Fr
(2)
Western Blot
(7)
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(7)
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(1)
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(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF3437 Applications Species
McCauley HA, Riedman AM, Enriquez JR et al. Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients Cellular and Molecular Gastroenterology and Hepatology 2023-01-04 [PMID: 36608902]
JR Enriquez, HA McCauley, KX Zhang, JG Sanchez, GT Kalin, RA Lang, JM Wells A dietary change to a high-fat diet initiates a rapid adaptation of the intestine Cell Reports, 2022-11-15;41(7):111641. 2022-11-15 [PMID: 36384107] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Julia A. Brown, Katherine Z. Sanidad, Serena Lucotti, Carolin M. Lieber, Robert M. Cox, Aparna Ananthanarayanan et al. Gut microbiota-derived metabolites confer protection against SARS-CoV-2 infection Gut Microbes 2022-12-31 [PMID: 35915556]
Rayan Khaddaj-Mallat, Natija Aldib, Maxime Bernard, Anne-Sophie Paquette, Aymeric Ferreira, Sarah Lecordier et al. SARS-CoV-2 deregulates the vascular and immune functions of brain pericytes via Spike protein Neurobiology of Disease 2021-12-01 [PMID: 34780863]
Suchitra Kamle, Bing Ma, Chuan Hua He, Bedia Akosman, Yang Zhou, Chang-Min Lee et al. Chitinase 3-like-1 is a therapeutic target that mediates the effects of aging in COVID-19 JCI Insight 2021-11-08 [PMID: 34747367]
K Bilinska, CS von Barthe, R Butowt Expression of the ACE2 Virus Entry Protein in the Nervus Terminalis Reveals the Potential for an Alternative Route to Brain Infection in COVID-19 Frontiers in Cellular Neuroscience, 2021-07-05;15(0):674123. 2021-07-05 [PMID: 34290590] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Reut Falach, Liat Bar-On, Shlomi Lazar, Tamar Kadar, Ohad Mazor, Moshe Aftalion et al. Mice with induced pulmonary morbidities display severe lung inflammation and mortality following exposure to SARS-CoV-2 JCI Insight 2021-06-22 [PMID: 33974566]
Neil Dani, Rebecca H. Herbst, Cristin McCabe, Gilad S. Green, Karol Kaiser, Joshua P. Head et al. A cellular and spatial map of the choroid plexus across brain ventricles and ages Cell 2021-05-01 [PMID: 33932339]
Vegi Naidu, Amir A Zeki, Pawan Sharma Sex Differences in the Induction of Angiotensin Converting Enzyme 2 (Ace-2) in Mouse Lungs after E-Cigarette Vapor Exposure and Its Relevance to Covid-19 Journal of Investigative Medicine 2021-06-01 [PMID: 33926967]
K Bilinska, CS von Barthe, R Butowt Expression of the ACE2 virus entry protein in the nervus terminalis suggests an alternative route for brain infection in COVID-19 bioRxiv : the preprint server for biology, 2021-06-07;0(0):. 2021-06-07 [PMID: 33880469] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Show All 19 Publications.

Review for ACE-2 Antibody (AF3437) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3437:
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WB
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Mouse 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22777933
SpeciesMouse

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Secondary Antibodies

 

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By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.
Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol ACE2
Uniprot