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15-PGDH/HPGD Antibody (016)

Images

 
Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, ...read more
Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated , stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
016
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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15-PGDH/HPGD Antibody (016) Summary

Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human 15-PGDH/HPGD (Accession#: NP_000851.2; Met1-Gln266).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HPGD
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for 15-PGDH/HPGD Antibody (016)

  • 15-hydroxyprostaglandin dehydrogenase [NAD+]
  • 15PGDH
  • 15-PGDH
  • EC 1.1.1
  • EC 1.1.1.141
  • HPGD
  • hydroxyprostaglandin dehydrogenase 15-(NAD)
  • NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
  • PGDH1
  • PGDH1PGDH
  • Prostaglandin dehydrogenase 1
  • SDR36C1
  • short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HPGD