Choose from nearly 1,000 secondary antibodies conjugated to more than 40 different enzymatic, fluorescent, or biotin labels. Use the filters on the left to sort our secondary antibodies by species, application, host, or conjugate.
To learn more about secondary antibodies, download and read our Secondary Antibody Handbook for technical information and tips to guide you when choosing a secondary antibody for your experiment.
To understand more about direct vs. indirect detection, including benefits of a secondary antibody and when to use one, please read our resources on Direct vs. Indirect Detection Methods.
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7157 | |||
| Allophycocyanin | NB7156APC | |||
| Biotin | NB7158 | |||
| DyLight 350 | NB7156UV | |||
| DyLight 405 | NB7156V | |||
| DyLight 488 | NB7156G | |||
| DyLight 550 | NB7156R | |||
| DyLight 594 | NB7156DL594 | |||
| DyLight 650 | NB7156C | |||
| DyLight 680 | NB7156FR | |||
| DyLight 755 | NB7156IR | |||
| FITC | NB7159 | |||
| PE | NB7156PE | |||
| PerCP | NB7156PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7157 | |||
| Allophycocyanin | NB7156APC | |||
| Biotin | NB7158 | |||
| DyLight 350 | NB7156UV | |||
| DyLight 405 | NB7156V | |||
| DyLight 488 | NB7156G | |||
| DyLight 550 | NB7156R | |||
| DyLight 594 | NB7156DL594 | |||
| DyLight 650 | NB7156C | |||
| DyLight 680 | NB7156FR | |||
| DyLight 755 | NB7156IR | |||
| FITC | NB7159 | |||
| HRP | NB7160 | |||
| PE | NB7156PE | |||
| PerCP | NB7156PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7536 | |||
| Allophycocyanin | NB7535APC | |||
| Biotin | NB7537 | |||
| DyLight 350 | NB7535UV | |||
| DyLight 405 | NB7535V | |||
| DyLight 488 | NB7535G | |||
| DyLight 550 | NB7535R | |||
| DyLight 594 | NB7535DL594 | |||
| DyLight 650 | NB7535C | |||
| DyLight 680 | NB7535FR | |||
| DyLight 755 | NB7535IR | |||
| FITC | NB7538 | |||
| HRP | NB7539 | |||
| PE | NB7535PE | |||
| PerCP | NB7535PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7240APC | |||
| Biotin | NB7240B | |||
| DyLight 350 | NB7240UV | |||
| DyLight 405 | NB7240V | |||
| DyLight 488 | NB7240G | |||
| DyLight 550 | NB7240R | |||
| DyLight 594 | NB7240DL594 | |||
| DyLight 650 | NB7240C | |||
| DyLight 680 | NB7240FR | |||
| DyLight 755 | NB7240IR | |||
| FITC | NB7241 | |||
| HRP | NB7242 | |||
| PE | NB7240PE | |||
| PerCP | NB7240PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75294 | |||
| Biotin | NBP1-75295 | |||
| DyLight 350 | NBP1-76054 | |||
| DyLight 488 | NBP1-75299 | |||
| DyLight 550 | NBP1-76055 | |||
| DyLight 594 | NBP1-76056 | |||
| DyLight 633 | NBP1-76057 | |||
| DyLight 650 | NBP1-76058 | |||
| FITC | NBP1-75296 | |||
| HRP | NBP1-75297 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-22358 | |||
| DyLight 350 | NB7237UV | |||
| DyLight 405 | NB7237V | |||
| DyLight 488 | NB7237G | |||
| DyLight 550 | NB7237R | |||
| DyLight 594 | NB7237DL594 | |||
| DyLight 650 | NB7237C | |||
| DyLight 680 | NB7237FR | |||
| DyLight 755 | NB7237IR | |||
| FITC | NB7237F | |||
| HRP | NB7238 | |||
| PE | NB7237PE | |||
| PerCP | NB7237PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75281 | |||
| DyLight 350 | NBP1-75635 | |||
| DyLight 488 | NBP1-75285 | |||
| DyLight 550 | NBP1-75636 | |||
| DyLight 594 | NBP1-75637 | |||
| DyLight 633 | NBP1-75638 | |||
| FITC | NBP1-75282 | |||
| HRP | NBP1-75283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75281 | |||
| Pre-adsorbed | NBP1-75282 | |||
| Pre-adsorbed | NBP1-75283 | |||
| Pre-adsorbed | NBP1-75285 | |||
| Pre-adsorbed | NBP1-75635 | |||
| Pre-adsorbed | NBP1-75636 | |||
| Pre-adsorbed | NBP1-75637 | |||
| Pre-adsorbed | NBP1-75638 | |||
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the secondary antibody is present). Flow cytometry image submitted by a verified customer review.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7156-img0001.jpg "Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the secondary antibody is present). Flow cytometry image submitted by a verified customer review.. Using the FITC format of this antibody.")
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Image using the HRP format of Goat anti-Mouse IgG (H+L) Secondary Antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB7535-img0010.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Image using the HRP format of Goat anti-Mouse IgG (H+L) Secondary Antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by Goat anti-Mouse IgG (H+L) Secondary Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7535-img0008.jpg)
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody Goat anti-Mouse IgG (H+L) Secondary Antibody used at a dilution of 1:200 (5ug/ml). Detection: DAB. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunohistochemistry-Paraffin-NB7535-img0009.jpg)
![Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC conjugated secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7535-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0021.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Also shown in this 2-color STED image is a-tubulin monoclonal antibody [MOUSE] detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody (colored RED). Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4C. Secondary antibody: DyLight 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0018.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72732-img0026.jpg)
![SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - Non-Reduced.Lane 2: Goat anti-Rabbit IgG - Reduced.Load: 1.0 ug per lane.Predicted/Observed size: 25 & 55 kDa - Reduced, 160 kDa - Non-Reduced for Goat IgG.Other Band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-SDS-Page-NBP1-72732-img0025.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0007.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of Goat anti-Rabbit IgG (H+L) Secondary Antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Analysis of WNV-E in A549 cell lysate using Goat anti-Rabbit IgG (H+L) Secondary Antibody. The secondary antibody was diluted in 5% milk at a dilution of 1:3000. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0004.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72739-img0005.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy and high resolution Stimulated Emission Depletion (STED) Microscopy. Both Dylight and Atto conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit C. (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0002.jpg)
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Staining of paraffin embedded mouse tissue. Goat anti-Mouse DyLight 488 was used as a secondary antibody. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Paraffin-NBP1-72739-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy (upper images) and high resolution Stimulated Emission Depletion (STED) Microscopy (lower images). Both Dylight and ATTO conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0001.jpg)
![Immunohistochemistry-Frozen: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - MDA-MB-231 cells were stained. Goat anti-Mouse DyLight 488 was used as a secondary antibody and DRAQ5 was used for nuclear stain. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-72739-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb, Rt, Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated.Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at 4C.Secondary antibody: N/A.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT. Image using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0006.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - A three-fold serial dilution of Mouse IgG starting at 200ug was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto 1 Hour at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0009.jpg)
![ELISA: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - ELISA results of purified Goat anti-Mouse IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Gt-anti-Donkey HRP conjugate 616-1302, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72739-img0011.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0002.jpg "Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.")
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies and analysed by FACS. Strategy to identify B cells based on CD3- MHCII+ and IgG+ staining. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep37796), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0001.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415395915.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534359.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175281.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241535683.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Intracellular & surface Ig staining of P. alecto splenocytes. Dead cells were excluded using Live/Dead eFluor 506 dye & live cells were gated for singlets using forward scatter height (FSC-H) & area (FSC-A). For all the staining approaches (surface only, intracellular only, or surface + intracellular), a distinct sIg+ CD3+/− population is observed. Greater than 85% of this population is MHCII+. Comparable percentages of these cells were obtained with surface staining only vs. intracellular staining only, thus strongly suggesting that this population is truly a sIg+ B cell population. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175251.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345310.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415284518.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534323.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371931.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345349.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241537191.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415392567.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291918.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291938.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175290.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345340.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241539458.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371920.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291963.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241536374.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415363745.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371966.jpg)
![Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72763-img0009.jpg "Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.")
![ELISA: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - ELISA Results of Purified Guinea Porcine Anti-Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 ug of Rabbit IgG [Red Line], Human IgG [Green Line], Goat IgG [Blue Line], Mouse IgG [Purple Line]. The working dilution is 1:17,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Guinea Porcine mx10 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72763-img0010.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0006.jpg "Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.")
![Immunohistochemistry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of mouse tissue. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-NBP1-75279-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human pancreatic cancer cells. Note: Need to be careful about bleeding into different filters since it might interfere with FITC. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75279-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human osteosarcoma cell line. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0005.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)