Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: ELISA, ICC/IF, IF/IHC, IHC-P, Immunohistochemistry, WB, Western Blot.

Showing Publications 1 - 10 of 45. Show All 45 Publications. Collapse Publications.

Publications using NB7539	Applications	Species
Lau, HC;Passalacqua, I;Jung, JH;Kwon, Y;Zocco, D;Park, SS;Oh, SW; Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry Scientific reports 2024-01-02 [PMID: 38167556]
Jiang WI, De Belly H, Wang B et al. Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin bioRxiv 2023-07-29 [PMID: 37546737]
Carmi Y, Khamaisi H, Adawi R et al. Secreted soluble factors from tumor-activated mesenchymal stromal cells confer platinum chemoresistance to ovarian cancer cells bioRxiv 2023-02-27 [PMID: 37175439] (WB)	WB
Carmi Y, Agbarya A, Khamaisi H et al. Ascites in malignant ovarian cancer confer ovarian cancer cells chemoresistance to platinum. Combination treatment with crizotinib and 2-hydroxyestradiol restores platinum sensitivity bioRxiv 2023-09-25 (WB, Human)	WB	Human
Chauhan NR, Kundu S, Bal R et al. Transgenic mouse models support a protective role of type I IFN response in SARS-CoV-2 infection-related lung immunopathology and neuroinvasion Cell reports 2023-10-23 [PMID: 37874678] (WB)	WB
Lee K, Rader N, Miller O et al. Intranasal VLP-RBD vaccine adjuvanted with BECC470 confers immunity against Delta SARS-CoV-2 challenge in K18-hACE2-mice bioRxiv 2023-04-26 [PMID: 37407405] (ELISA) Details: Dilution: 1:10000	ELISA
Kumar R, Mehta D, Nayak D, Sunil S Characterization of an Aedes ADP-Ribosylation Protein Domain and Role of Post-Translational Modification during Chikungunya Virus Infection Pathogens (Basel, Switzerland) 2023-05-16 [PMID: 37242388] (WB)	WB
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (ICC/IF, WB)	ICC/IF, WB
Evans JG, Novotny LA, Meissner EG Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling Viruses 2023-02-25 [PMID: 36992341] (WB)	WB
Swirski S, May O, Ahlers M et al. In Vivo Efficacy and Safety Evaluations of Therapeutic Splicing Correction Using U1 snRNA in the Mouse Retina Cells 2023-03-21 [PMID: 36980294] (WB)	WB
Owczarek-Lipska M, Markus F, B�ltmann E et al. A TARP Syndrome Phenotype Is Associated with a Novel Splicing Variant in RBM10 Genes (Basel) 2022-11-18 [PMID: 36421828] (Western Blot)	Western Blot
DuCote T Targeting EZH2 to Improve Outcomes of Lung Squamous Cell Carcinoma uknowledge.uky.edu 2023-01-01 (WB)	WB
Kumar R, Mehta D, Chaudhary S Et al. Impact of CHIKV Replication on the Global Proteome of Aedes albopictus Cells Proteomes 2022-11-22 [PMID: 36412637] (WB) Details: Citation using the HRP version of this antibody.	WB
Li H, Zhao T, Cao F et al. Integrated bioactive scaffold with aptamer targeted stem cell recruitment and growth factor induced pro differentiation effects for anisotropic meniscal regeneration Bioengineering & Translational Medicine 2022-03-03 [PMID: 36176622] (IHC-P)	IHC-P
Yang Z, Cao F, Li H Et al. Microenvironmentally optimized 3D-printed TGFb-functionalized scaffolds facilitate endogenous cartilage regeneration in sheep Acta Biomater 2022-07-27 [PMID: 35896136] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Marino M, Zhou L, Rincon MY Et al. AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model EMBO Mol Med 2022-03-30 [PMID: 35352880] (WB) Details: Citation using the HRP version of this antibody.	WB
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429] Details: Citation using the HRP version of this antibody.
Khamaisi H, Mahmoud H, Mahajna J. Et al. 2-Hydroxyestradiol Overcomes Mesenchymal Stem Cells-Mediated Platinum Chemoresistance in Ovarian Cancer Cells in an ERK-Independent Fashion Molecules 2022-02-15 [PMID: 35164068] (WB) Details: Citation using the HRP version of this antibody.	WB
Abdel-Bakky MS, Alqasoumi A, Altowayan WM Et al. Simvastatin mitigates streptozotocin-induced type 1 diabetes in mice through downregulation of ADAM10 and ADAM17 Life Sci 2021-12-13 [PMID: 34896343] Details: Citation using the HRP version of this antibody.
JUschke C, Klopstock T, Catarino CB Et al. Autosomal dominant optic atrophy: A novel treatment for OPA1 splice defects using U1 snRNA adaption Mol Ther Nucleic Acids 2021-12-02 [PMID: 34853716] (WB) Details: Citation using the HRP version of this antibody.	WB
Markus F, Kannengieber A, NAder P Et al. A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects J Hum Genet 2021-07-02 [PMID: 34211111] Details: Citation using the HRP version of this antibody.
Knapp-Wilson A, Pereira GC, Buzzard E et al. Maintenance of complex I and its supercomplexes by NDUF-11 is essential for mitochondrial structure, function and health J Cell Sci 2021-06-09 [PMID: 34106255] (WB) Details: This citation used the HRP format of this antibody.	WB
Yang Z, Li H, Tian Y et al. Biofunctionalized Structure and Ingredient Mimicking Scaffolds Achieving Recruitment and Chondrogenesis for Staged Cartilage Regeneration Frontiers in Cell and Developmental Biology 2021-03-25 [PMID: 33842484] (Immunohistochemistry)	Immunohistochemistry
Padder RA, Bhat ZI, Ahmad Z et al. DRP1 Promotes BRAF(V600E)-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer Frontiers in Oncology 2021-03-02 [PMID: 33738242] (Western Blot)	Western Blot
Baena G, Feria AB, HernAndez-Huertas L et al Genetic and Pharmacological Inhibition of Autophagy increases the Monoubiquitination of Non-Photosynthetic Phosphoenolpyruvate Carboxylase Plants (Basel) 2020-12-30 [PMID: 33374865] Details: Citation using the HRP version of this antibody.
Koren Carmi Y, Mahmoud H, Khamaisi H et al. Flavonoids Restore Platinum Drug Sensitivity to Ovarian Carcinoma Cells in a Phospho-ERK1/2-Dependent Fashion International Journal of Molecular Sciences 2020-09-07 [PMID: 32906729]
Park SH, Lee DH, Choi HI et al. Synergistic lipid-lowering effects of Zingiber mioga and Hippophae rhamnoides extracts Experimental and Therapeutic Medicine 2020-06-19 [PMID: 32765704]
G�mez-Caudillo L, Ortega-Lozano AJ, Mart�nez-Batallar �G et al. Principal component analysis on LC?MS/MS and 2DE?MALDI?TOF in glioblastoma cell lines reveals that mitochondria act as organelle sensors of the metabolic state in glioblastoma Oncology Reports 2020-05-27 [PMID: 32468038] (Western Blot)	Western Blot
Md. Aejazur Rahman, Bridgette M. Cumming, Kelvin W. Addicott, Hayden T. Pacl, Shannon L. Russell, Kievershen Nargan, Threnesan Naidoo, Pratistadevi K. Ramdial, John H. Adamson, Rui Wang, Adrie J. C. Steyn Hydrogen sulfide dysregulates the immune response by suppressing central carbon metabolism to promote tuberculosis Proceedings of the National Academy of Sciences of the United States of America 2020-03-24 [PMID: 32139610]
Han Y, Sanford L, Simpson DM et al. Remodeling of Zn(2+) homeostasis upon differentiation of mammary epithelial cells Metallomics 2020-01-18 [PMID: 31950952] (WB)	WB
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol 2019-09-26 [PMID: 31551961] (WB)	WB
Katsumi T, Guicciardi ME, Azad A et al Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11. Am J Physiol Cell Physiol. 2019-10-01 [PMID: 31365294] Details: Citation using the HRP version of this antibody.
Alexander Shapson-Coe, Brenda Valeiras, Christopher Wall, Cristina Rada, Mary Bryk Aicardi–Goutières Syndrome associated mutations of RNase H2B impair its interaction with ZMYM3 and the CoREST histone-modifying complex PLoS ONE 2019-03-19 [PMID: 30889214]
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P)	IHC-P
Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics 2018-11-16 [PMID: 30429885] (IF/IHC)	IF/IHC
Wang X, Hybiske K, Stephens RS. Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells Pathog Dis 2018-02-02 [PMID: 29390129] (WB)	WB
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on beta-amyloid aggregation and degradation J Biol Chem 2017-10-20 [PMID: 29046353] (WB)	WB
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia Nat Commun 2016-12-16 [PMID: 27976675] (WB)	WB
Moghimi E, Solomon JA, Gianforcaro A et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis PLoS One 2015-05-29 [PMID: 26020962] (WB)	WB
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia 2013-06-13 [PMID: 23756832] (WB)	WB
Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet. [PMID: 22843495] Details: Citation using the HRP form of this antibody.
Lin YT, Ding JY, Li MY et al. YAP regulates neuronal differentiation through Sonic hedgehog signaling pathway. Exp Cell Res. [PMID: 22659622] Details: Citation using the HRP form of this antibody.
Kim HJ, Lim SJ, Kwag HL et al. The choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particles. PLoS One. 2012-01-01 [PMID: 22563414] Details: Citation using the HRP form of this antibody.
Soenawan E, Srivastava I, Gupta S et al. Maintenance of long-term immunological memory by low avidity IgM-secreting cells in bone marrow after mucosal immunizations with cholera toxin adjuvant. Vaccine. [PMID: 15063581] Details: Citation using the HRP form of this antibody.
Helske S, Vuorela P, Carpen O et al. Expression of vascular endothelial growth factor receptors 1, 2 and 3 in placentas from normal and complicated pregnancies. Mol Hum Reprod. [PMID: 11160848] Details: Citation using the HRP form of this antibody.
Show All 45 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Ming Lu	WB	Human	08/14/2014	View Summary Application Western Blot Sample Tested Human Pancreatic Cancer Cell line Species Human Lot A33

Array NB7539

• IgG (H+L) Antibodies