Goat anti-Mouse IgG (H+L) Secondary Antibody

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, MiAr, WB.

Showing Publications 1 - 10 of 42. Show All 42 Publications. Collapse Publications.

Publications using NB7535	Applications	Species
Brinch CM, Hogdall E, Heer P et al. The Prognostic Value of Plasma Small Extracellular Vesicles' Phenotype in Patients With Gastrointestinal Stromal Tumor Anticancer research 2022-12-01 [PMID: 36456119] (MiAr)	MiAr
Kumar R, Mehta D, Chaudhary S Et al. Impact of CHIKV Replication on the Global Proteome of Aedes albopictus Cells Proteomes 2022-11-22 [PMID: 36412637] (WB) Details: Citation using the HRP version of this antibody.	WB
Diez-Escudero A, Andersson B, Carlsson E Et al. 3D-printed porous Ti6Al4V alloys with silver coating combine osteocompatibility and antimicrobial properties Biomater Adv 2022-05-08 [PMID: 35527155] Details: Citation using the Biotin version of this antibody.
Khamaisi H, Mahmoud H, Mahajna J. Et al. 2-Hydroxyestradiol Overcomes Mesenchymal Stem Cells-Mediated Platinum Chemoresistance in Ovarian Cancer Cells in an ERK-Independent Fashion Molecules 2022-02-15 [PMID: 35164068] (WB) Details: Citation using the HRP version of this antibody.	WB
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC)	IF/IHC
Yang Z, Cao F, Li H Et al. Microenvironmentally optimized 3D-printed TGFb-functionalized scaffolds facilitate endogenous cartilage regeneration in sheep Acta Biomater 2022-07-27 [PMID: 35896136] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Marino M, Zhou L, Rincon MY Et al. AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model EMBO Mol Med 2022-03-30 [PMID: 35352880] (WB) Details: Citation using the HRP version of this antibody.	WB
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429] Details: Citation using the HRP version of this antibody.
Abdel-Bakky MS, Alqasoumi A, Altowayan WM Et al. Simvastatin mitigates streptozotocin-induced type 1 diabetes in mice through downregulation of ADAM10 and ADAM17 Life Sci 2021-12-13 [PMID: 34896343] Details: Citation using the HRP version of this antibody.
JUschke C, Klopstock T, Catarino CB Et al. Autosomal dominant optic atrophy: A novel treatment for OPA1 splice defects using U1 snRNA adaption Mol Ther Nucleic Acids 2021-12-02 [PMID: 34853716] (WB) Details: Citation using the HRP version of this antibody.	WB
Poojary KK, Nayak G, Vasani A Et al. Curcumin nanocrystals attenuate cyclophosphamide-induced testicular toxicity in mice Toxicol Appl Pharmacol 2021-10-29 [PMID: 34715073] (ICC/IF) Details: Citation using the FITC version of this antibody.	ICC/IF
Markus F, Kannengieber A, NAder P Et al. A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects J Hum Genet 2021-07-02 [PMID: 34211111] Details: Citation using the HRP version of this antibody.
Knapp-Wilson A, Pereira GC, Buzzard E et al. Maintenance of complex I and its supercomplexes by NDUF-11 is essential for mitochondrial structure, function and health J Cell Sci 2021-06-09 [PMID: 34106255] (WB) Details: This citation used the HRP format of this antibody.	WB
Zou Z, Liu R, Wang Y, et al. IL1RN promotes osteoblastic differentiation via interacting with ITGB3 in osteoporosis Acta biochimica et biophysica Sinica 2021-01-26 [PMID: 33493267]
Baena G, Feria AB, HernAndez-Huertas L et al Genetic and Pharmacological Inhibition of Autophagy increases the Monoubiquitination of Non-Photosynthetic Phosphoenolpyruvate Carboxylase Plants (Basel) 2020-12-30 [PMID: 33374865] Details: Citation using the HRP version of this antibody.
Meitei HY, Uppangala S, Sharan K et al. A Simple, Centrifugation-Free, Sperm-Sorting Device Eliminates the Risks of Centrifugation in the Swim-Up Method While Maintaining Functional Competence and DNA Integrity of Selected Spermatozoa Reprod Sci 2020-07-30 [PMID: 32734563] (ICC/IF)	ICC/IF
Hegde S, Poojary KK, Rasquinha R Et al. Epigallocatechin-3-gallate (EGCG) protects the oocytes from methyl parathion-induced cytoplasmic deformities by suppressing oxidative and endoplasmic reticulum stress Pestic Biochem Physiol 2020-06-13 [PMID: 32527428] Details: Citation using the FITC version of this antibody.
Ismail A, Doghish AS, E M Elsadek B et al. Hydroxycitric acid potentiates the cytotoxic effect of tamoxifen in MCF-7 breast cancer cells through inhibition of ATP citrate lyase Steroids 2020-05-23 [PMID: 32439410] (WB) Details: Citation using the Alkaline Phosphatase format of this antibody.	WB
Han Y, Sanford L, Simpson DM et al. Remodeling of Zn(2+) homeostasis upon differentiation of mammary epithelial cells Metallomics 2020-01-18 [PMID: 31950952] (WB)	WB
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol 2019-09-26 [PMID: 31551961] (WB)	WB
Katsumi T, Guicciardi ME, Azad A et al Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11. Am J Physiol Cell Physiol. 2019-10-01 [PMID: 31365294] Details: Citation using the HRP version of this antibody.
Elsadek BEM, Abdelghany AA, Abd El-Aziz MA et al Validation of the Diagnostic and Prognostic Values of ADAMTS5 and FSTL1 in Osteoarthritis Rat Model Cartilage. 2019-06-10 [PMID: 31177809] Details: Citation using the Alkaline Phosphatase version of this antibody.
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P)	IHC-P
Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics 2018-11-16 [PMID: 30429885] (IF/IHC)	IF/IHC
Kwon TR, Oh CT, Bak DH et al. Effects on skin of Stichopus japonicus viscera extracts detected with saponin including Holothurin A: Down-regulation of melanin synthesis and up-regulation of neocollagenesis mediated by ERK signaling pathway J Ethnopharmacol 2018-08-14 [PMID: 30102992] (ICC/IF)	ICC/IF
Wang X, Hybiske K, Stephens RS. Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells Pathog Dis 2018-02-02 [PMID: 29390129] (WB)	WB
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on beta-amyloid aggregation and degradation J Biol Chem 2017-10-20 [PMID: 29046353] (WB)	WB
Saribas AS, DeVoto J, Golla A Et al. Discovery and characterization of novel trans-spliced products of human polyoma JC virus late transcripts from PML patients J Cell Physiol 2017-10-19 [PMID: 29044559] Details: Citation using the Rhodamine version of this antibody.
England CG, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer Mol Pharm 2017-08-22 [PMID: 28825843] (FLOW, ICC/IF)	FLOW, ICC/IF
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia Nat Commun 2016-12-16 [PMID: 27976675] (WB)	WB
Kwon TR, Oh CT, Choi EJ et al. Human placental extract exerts hair growth-promoting effects through the GSK-3b signaling pathway in human dermal papilla cells. Int J Mol Med. [PMID: 26311045] (ICC/IF) Details: Citation using the FITC form of this antibody.	ICC/IF
Moghimi E, Solomon JA, Gianforcaro A et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis PLoS One 2015-05-29 [PMID: 26020962] (WB)	WB
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia 2013-06-13 [PMID: 23756832] (WB)	WB
Yoon J, Juhn KM, Ko JK et al. Effects of oxygen tension and IGF-I on HIF-1 alpha protein expression in mouse blastocysts J Assist Reprod Genet 2012-12-13 [PMID: 23232974] (IF/IHC, ICC/IF)	IF/IHC, ICC/IF
Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet. [PMID: 22843495] Details: Citation using the HRP form of this antibody.
Lin YT, Ding JY, Li MY et al. YAP regulates neuronal differentiation through Sonic hedgehog signaling pathway. Exp Cell Res. [PMID: 22659622] Details: Citation using the HRP form of this antibody.
Kim HJ, Lim SJ, Kwag HL et al. The choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particles. PLoS One. 2012-01-01 [PMID: 22563414] Details: Citation using the HRP form of this antibody.
Wilkerson et al. Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. Vet Immunol Immunopathol. [PMID: 15963817] (FLOW) Details: Citation using the FITC form of this antibody.	FLOW
Soenawan E, Srivastava I, Gupta S et al. Maintenance of long-term immunological memory by low avidity IgM-secreting cells in bone marrow after mucosal immunizations with cholera toxin adjuvant. Vaccine. [PMID: 15063581] Details: Citation using the HRP form of this antibody.
Fukumoto et al. Identification and expression of a 50-kilodalton surface antigen of Babesia gibsoni and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. J Clin Microbiol. [PMID: 11427577] Details: Citation using the FITC form of this antibody.
Helske S, Vuorela P, Carpen O et al. Expression of vascular endothelial growth factor receptors 1, 2 and 3 in placentas from normal and complicated pregnancies. Mol Hum Reprod. [PMID: 11160848] Details: Citation using the HRP form of this antibody.
Flaminio MJ, Rush BR, Davis EG et al. Characterization of peripheral blood and pulmonary leukocyte function in healthy foals. Vet Immunol Immunopathol. [PMID: 10713340] Details: Citation using the FITC form of this antibody.
Show All 42 Publications. Collapse Publications.

Array NB7535

• IgG (H+L) Antibodies