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Goat anti-Rabbit IgG (H+L) Secondary Antibody

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Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat anti-Rabbit IgG (H+L) Secondary Antibody Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:30000 (for coating plates use 1:100-1:500)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200-1:2000
  • Immunohistochemistry 1:200-1:2000
  • Immunohistochemistry-Paraffin 1:200-1:2000
  • Western Blot 1:1000-1:30000
Application Notes
Use in Flow Cytometry was reported in the scientific literature (PMID: 30710055).
Publications
Read Publications using NB7156.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7156)(51)

We have publications tested in 7 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(2)
FLOW
(1)
ICC/IF
(1)
IF/IHC
(4)
IHC-Fr
(1)
IHC-P
(5)
WB
(26)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using NB7156 Applications Species
Elmahmoudy B, Abd El Fattah M, Elyamany M et al. Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats Asian Pac J Trop Biomed 2019-10-01 (WB) WB
Khaled S, Abdelaziz RR, Suddek GM, Elshaer SL Candesartan protects against unilateral peripheral limb ischemia in type-2 diabetic rats: Possible contribution of PI3K-Akt-eNOS-VEGF angiogenic signaling pathway International immunopharmacology 2023-02-09 [PMID: 36773570] (WB) WB
El Assar M, GarcIa-Rojo E, Sevilleja-Ortiz A Et al. Functional Role of STIM-1 and Orai1 in Human Microvascular Aging Cells 2022-11-26 [PMID: 36429103] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P)

Details:
Citation using the HRP version of this antibody.
IHC-P
Zwida KH, Kutzler MA. Canine splenic hemangiosarcoma cells express and activate luteinizing hormone receptors in vitro Am J Vet Res 2022-11-03 [PMID: 36327166] (ICC/IF) ICC/IF
Hermanns N, Wroblewski V, BascuNana P Et al. Molecular imaging of the brain-heart axis provides insights into cardiac dysfunction after cerebral ischemia Basic Res Cardiol 2022-10-24 [PMID: 36279013] (IHC-Fr)

Details:
Citation using the Biotin version of this antibody.
IHC-Fr
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Swamy G, Salo P, Duncan N Et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice JOR Spine 2022-07-05 [PMID: 35783913] (IHC-P)

Details:
Citation using the HRP version of this antibody.
IHC-P
Hendawy AS, El-Lakkany NM, Mantawy EM Et al. Vildagliptin alleviates liver fibrosis in NASH diabetic rats via modulation of insulin resistance, oxidative stress, and inflammatory cascades Life Sci 2022-06-07 [PMID: 35671811] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Salem MA, Manaa EG, Osama N Et al. Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFb/SMAD pathway Sci Rep 2022-04-22 [PMID: 35449437] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Show All 51 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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