Goat anti-Rabbit IgG (H+L) Secondary Antibody

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 7 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 51. Show All 51 Publications. Collapse Publications.

Publications using NB7156	Applications	Species
Elmahmoudy B, Abd El Fattah M, Elyamany M et al. Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats Asian Pac J Trop Biomed 2019-10-01 (WB)	WB
Khaled S, Abdelaziz RR, Suddek GM, Elshaer SL Candesartan protects against unilateral peripheral limb ischemia in type-2 diabetic rats: Possible contribution of PI3K-Akt-eNOS-VEGF angiogenic signaling pathway International immunopharmacology 2023-02-09 [PMID: 36773570] (WB)	WB
El Assar M, GarcIa-Rojo E, Sevilleja-Ortiz A Et al. Functional Role of STIM-1 and Orai1 in Human Microvascular Aging Cells 2022-11-26 [PMID: 36429103] (WB) Details: Citation using the HRP version of this antibody.	WB
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Zwida KH, Kutzler MA. Canine splenic hemangiosarcoma cells express and activate luteinizing hormone receptors in vitro Am J Vet Res 2022-11-03 [PMID: 36327166] (ICC/IF)	ICC/IF
Hermanns N, Wroblewski V, BascuNana P Et al. Molecular imaging of the brain-heart axis provides insights into cardiac dysfunction after cerebral ischemia Basic Res Cardiol 2022-10-24 [PMID: 36279013] (IHC-Fr) Details: Citation using the Biotin version of this antibody.	IHC-Fr
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB) Details: Citation using the HRP version of this antibody.	WB
Swamy G, Salo P, Duncan N Et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice JOR Spine 2022-07-05 [PMID: 35783913] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Hendawy AS, El-Lakkany NM, Mantawy EM Et al. Vildagliptin alleviates liver fibrosis in NASH diabetic rats via modulation of insulin resistance, oxidative stress, and inflammatory cascades Life Sci 2022-06-07 [PMID: 35671811] (WB) Details: Citation using the HRP version of this antibody.	WB
Salem MA, Manaa EG, Osama N Et al. Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFb/SMAD pathway Sci Rep 2022-04-22 [PMID: 35449437] (WB) Details: Citation using the HRP version of this antibody.	WB
Kortam MA, Alawady AS, Hamid Sadik NA Et al. Fenofibrate mitigates testosterone induced benign prostatic hyperplasia via regulation of Akt/FOXO3a pathway and modulation of apoptosis and proliferation in rats Arch Biochem Biophys 2022-04-17 [PMID: 35430215] (WB) Details: Citation using the HRP version of this antibody.	WB
Wang L, Zhang X, Liu J Et al. MUC21 induces the viability and migration of glioblastoma via the STAT3/AKT pathway Exp Ther Med 2022-04-11 [PMID: 35401801] (IF/IHC) Details: Citation using the Biotin version of this antibody.	IF/IHC
Wu X, Pang Q, Zhi F Et al. Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells Tissue Cell 2022-03-24 [PMID: 35325674] (WB) Details: Citation using the HRP version of this antibody.	WB
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429] Details: Citation using the HRP version of this antibody.
Abdel-Latif RT, Wadie W, Abdel-Mottaleb Y Et al. Reposition of the anti-inflammatory drug diacerein in an in-vivo colorectal cancer model Saudi Pharm J 2022-02-11 [PMID: 35145347] (WB) Details: Citation using the HRP version of this antibody.	WB
Marchini M, Ashkin MR, Bellini M Et al. A Na(+)/K(+) ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice Front Cell Dev Biol 2021-12-31 [PMID: 34970538] (IF/IHC) Details: Citation using the HRP version of this antibody.	IF/IHC
Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mech Ageing Dev 2021-10-26 [PMID: 34699858] (WB) Details: Citation using the HRP version of this antibody.	WB
Chen F, Liu J, Song X Et al. EZH2 inhibition confers PIK3CA-driven lung tumors enhanced sensitivity to PI3K inhibition Cancer Lett 2021-10-16 [PMID: 34655667] (WB) Details: Citation using the HRP version of this antibody.	WB
Markus F, Kannengieber A, NAder P Et al. A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects J Hum Genet 2021-07-02 [PMID: 34211111] Details: Citation using the HRP version of this antibody.
Abdel-Fattah MM, Elgendy ANAM, Mohamed WR. Xanthenone, ACE2 activator, counteracted gentamicin-induced nephrotoxicity in rats: Impact on oxidative stress and ACE2/Ang-(1-7) signaling Life Sci 2021-03-28 [PMID: 33774027] (WB) Details: Citation using the HRP format of this antibody.	WB
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (IHC-P) Details: Citation using the Alexa Fluor 488 version of this antibody.	IHC-P
Iguchi N, Carrasco A, Xie AX, et al Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways Sci Rep 2021-01-14 [PMID: 33441874] (WB) Details: Citation using the HRP version of this antibody.	WB
Ogura Y, Kakehashi C, Yoshihara T et al Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats PLoS One 2020-10-30 [PMID: 33125406] (WB) Details: Citation using the HRP version of this antibody.	WB
Harb I, Ashour H, Sabry D et al. Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1alpha/VEGF /eNOS signaling J. Pharm. Pharmacol. 2020-08-21 [PMID: 32822562] Details: Citation using the HRP format of this antibody.
S Kaji, T Maki, J Ueda, T Ishimoto, Y Inoue, K Yasuda, M Sawamura, R Hikawa, T Ayaki, H Yamakado, R Takahashi BCAS1-positive immature oligodendrocytes are affected by the &alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun, 2020-07-29;8(1):120. 2020-07-29 [PMID: 32727582] (WB)	WB
La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. 2020-07-23 [PMID: 32712093] (WB) Details: Citation using the HRP format of this antibody.	WB
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun 2020-05-29 [PMID: 32472050] (WB) Details: Citation using the HRP format of this antibody.	WB
Ismail A, Doghish AS, E M Elsadek B et al. Hydroxycitric acid potentiates the cytotoxic effect of tamoxifen in MCF-7 breast cancer cells through inhibition of ATP citrate lyase Steroids 2020-05-23 [PMID: 32439410] (WB) Details: Citation using the Alkaline Phosphatase format of this antibody.	WB
AbuBakr N, Haggag T, Sabry D et al. Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGF beta Smad3 signaling pathway Heliyon 2020-05-09 [PMID: 32382678] (WB)	WB
Choi JW, Lee SK, Kim MJ et al. Piperine ameliorates the severity of fibrosis via inhibition of TGF-beta SMAD signaling in a mouse model of chronic pancreatitis Mol Med Rep 2019-09-06 [PMID: 31485676] (WB)	WB
Mehta N, Maddineni S, Mathews II et al. Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation Cell Rep. 2019-09-03 [PMID: 31484064] (ELISA) Details: Citation used the HRP format of this antibody.	ELISA
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res 2019-08-05 [PMID: 31377485] (WB)	WB
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal 2019-07-06 [PMID: 31273575] (WB)	WB
Gao Q, Yang Z, Xu S et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer J Exp Med 2019-02-03 [PMID: 30710055] (FLOW)	FLOW
Schilling BK, Schusterman MA 2nd, Kim DY et al. Adipose-derived stem cells delay muscle atrophy after peripheral nerve injury in the rodent model Muscle Nerve 2019-01-26 [PMID: 30681163] (IF/IHC)	IF/IHC
Yang T, Moore M, He F Pten regulates neural crest proliferation and differentiation during mouse craniofacial development Dev Dyn. 2018-02-01 [PMID: 29115005] Details: Citation using the Alkaline Phosphatase form of this antibody.
Almalki SG, Llamas Valle Y, Agrawal DK. MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells Stem Cells Transl Med 2017-02-19 [PMID: 28213979] (WB)	WB
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12 1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun. [PMID: 27384883] (WB) Details: Citation using the HRP form of this antibody.	WB
Besingi RN, Clark PL. Extracellular protease digestion to evaluate membrane protein cell surface localization. Nat Protoc. [PMID: 26584447] Details: Citation using the Alkaline Phosphatase form of this antibody.
Lucarelli G, Galleggiante V, Rutigliano M et al. Metabolomic profile of glycolysis and the pentose phosphate pathway identifies the central role of glucose-6-phosphate dehydrogenase in clear cell-renal cell carcinoma. Oncotarget. [PMID: 25945836] (IHC-P) Details: Citation using the FITC form of this antibody.	IHC-P
Hur J, Kim CS, Eo SK et al. Salmonella ghosts expressing enterotoxigenic Escherichia coli k88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model. Vet Q. [PMID: 25853619] (ELISA) Details: Citation using the HRP form of this antibody.	ELISA
Korajoki H, Vornanen M. Species- and chamber-specific responses of 12 kDa FK506-binding protein to temperature in fish heart Fish Physiol Biochem 2013-09-20 [PMID: 24048915] (WB)	WB
Korajoki H, Vornanen M. Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts J Comp Physiol B 2012-12-15 [PMID: 23239195] (WB)	WB
Pitcher MH, Nieto FR, Cervero F. Stimulation of cutaneous low threshold mechanoreceptors in mice after intracolonic capsaicin increases spinal c-Fos labeling in an NKCC1-dependent fashion J Pain 2012-12-04 [PMID: 23199419] (IF/IHC)	IF/IHC
Fujii S, Hara H, Araya J et al. Insufficient autophagy promotes bronchial epithelial cell senescence in chronic obstructive pulmonary disease. Oncoimmunology. [PMID: 22934255] Details: Citation using the HRP form of this antibody.
Lu Y, Kim C, Collins JF et al. Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes. J Trace Elem Med Biol. [PMID: 22579041] Details: Citation using the HRP form of this antibody.
Ganburged G, Suda N, Saito M et al. Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice Cell Tissue Res 2010-08-18 [PMID: 20714769] (IHC-P)	IHC-P
Dickinson GH, Vega IE, Wahl KJ et al. Barnacle cement: a polymerization model based on evolutionary concepts. J Exp Biol. [PMID: 19837892] Details: Citation using the HRP form of this antibody.
Wang H, Kubica N, Ellisen LW et al. Dexamethasone represses signaling through the mammalian target of rapamycin in muscle cells by enhancing expression of REDD1. J Biol Chem. [PMID: 17074751] Details: Citation using the HRP form of this antibody.
Kimball SR, Siegfried BA, Jefferson LS et al. Glucagon represses signaling through the mammalian target of rapamycin in rat liver by activating AMP-activated protein kinase. J Biol Chem. [PMID: 15494402] Details: Citation using the HRP form of this antibody.
Roesler U, Scholz H, Hensel A et al. Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Int J Syst Evol Microbiol. [PMID: 12892149] Details: Citation using the HRP form of this antibody.
Show All 51 Publications. Collapse Publications.

Array NB7156

• IgG (H+L) Antibodies