Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 application: WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP1-75297	Applications	Species
Kwon J, Yeh Y, Kawarasaki S et al. Mevalonate biosynthesis pathway regulates the development and survival of brown adipocytes iScience 2023-03-01 [PMID: 36895651] (WB)	WB
Nadeem RI, Aboutaleb AS, Younis NS, Ahmed HI Diosmin Mitigates Gentamicin-Induced Nephrotoxicity in Rats: Insights on miR-21 and -155 Expression, Nrf2/HO-1 and p38-MAPK/NF-?B Pathways Toxics 2023-01-01 [PMID: 36668774] (WB) Details: 1:2000 WB dilution	WB
Xu W, Tang J, Zhao L DNA-protein cross-links between abasic DNA damage and mitochondrial transcription factor A (TFAM) Nucleic acids research 2022-12-30 [PMID: 36583367] (WB) Details: Dilution used in WB 1:1000	WB
Ng SP, Nomura W, Takahashi H Et al. Methylglyoxal induces multiple serine phosphorylation in insulin receptor substrate 1 via the TAK1-p38-mTORC1 signaling axis in adipocytes Biochem J 2022-10-18 [PMID: 36256829] Details: Citation using the HRP version of this antibody.
Ng SP, Nomura W, Takahashi H Et al. Methylglyoxal attenuates isoproterenol-induced increase in uncoupling protein 1 expression through activation of JNK signaling pathway in beige adipocytes Biochem Biophys Rep 2021-09-16 [PMID: 34527816] (WB) Details: Citation using the HRP version of this antibody.	WB
Gomaa AA, El-Abhar HS, Abdallah DM et al. Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-I kappa B alpha/Ubc9 and SIRT-1/miR-22 trajectories Toxicol Appl Pharmacol 2021-06-26 [PMID: 34174262] Details: This citation used the HRP format of this antibody.
Salem MA, Ismail RS, Zaki HF et al. L-carnitine extenuates endocrine disruption, inflammatory burst and oxidative stress in carbendazim-challenged male rats via upregulation of testicular StAR and FABP9, and downregulation of P38-MAPK pathways Toxicology 2021-05-09 [PMID: 33965443] Details: Citation using the HRP format of this antibody.
Amano H, Mastui Y, Ito Y et al. The role of vascular endothelial growth factor receptor 1 tyrosine kinase signaling in bleomycin-induced pulmonary fibrosis Biomed Pharmacother 2019-06-09 [PMID: 31176171] (WB)	WB
Khedr RM, Ahmed AAE, Kamel R et al. Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner Life Sci 2018-09-09 [PMID: 30195035] (WB, WB)	WB, WB
Luo B, Wang J, Liu Z et al. Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution. Nat Commun. [PMID: 27397585] (WB) Details: Citation using the HRP form of this antibody.	WB
Luo B, Jiang M, Yang X et al. Erythropoietin is a hypoxia inducible factor-induced protective molecule in experimental autoimmune neuritis Biochim Biophys Acta 2013-04-23 [PMID: 23603807] (WB)	WB
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	11/12/2014	View Summary Application Western Blot Sample Tested A549 whole cell lysate Species Human

Array NBP1-75297

• IgG (H+L) Antibodies