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![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Western-Blot-NB100-56725-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.")
![Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Immunohistochemistry-Paraffin-NB100-56725-img0002.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0011.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Western-Blot-NB100-56725-img0005.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug antibody/test/1 million cells) and ((as seen in B). this is because (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0004.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells using 0.5 ug of NB100-56060. The shaded histogram represents cells without antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0009.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated (right) Ramos cells using 0.5 ug of was used for this test.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0010.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Western-Blot-NB100-56556-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.")
![Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Flow-Cytometry-NB100-56556-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Western-Blot-NB100-56556-img0002.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody [NBP2-33608] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-Western-Blot-NBP2-33608-img0002.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody [NBP2-33608] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody [NBP2-33608] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-Immunohistochemistry-Paraffin-NBP2-33608-img0001.jpg)
![Immunohistochemistry;D4-GDI/RhoGDI2 Antibody[NBP3-29103]Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human breast carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI used at a dilution of 1:5,000 0.2 µg/ml](http://images.novusbio.com/fullsize/nbp3-29104_rabbit-d4-gdi-rhogdi2-pab-78202410153514.jpg "Immunohistochemistry;D4-GDI/RhoGDI2 Antibody[NBP3-29103]Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human breast carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI used at a dilution of 1:5,000 0.2 µg/ml")
![Western Blot: D4-GDI/RhoGDI2 Antibody [H00000397-B01P] - Analysis of ARHGDIB expression in human spleen.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-Western-Blot-H00000397-B01P-img0003.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody [H00000397-B01P] - Analysis of ARHGDIB expression in human spleen.")
![Western Blot: D4-GDI/RhoGDI2 Antibody [H00000397-B01P] - Analysis of ARHGDIB expression in transfected 293T cell line by ARHGDIB polyclonal antibody. Lane 1: ARHGDIB transfected lysate(22.11 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-Western-Blot-H00000397-B01P-img0004.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody [NBP2-92431] - Analysis of extracts of various cell lines, using D4-GDI/RhoGDI2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-Western-Blot-NBP2-92431-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody [NBP2-92431] - Analysis of extracts of various cell lines, using D4-GDI/RhoGDI2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![SDS-Page: Recombinant Human D4-GDI/RhoGDI2 Protein [NBP1-99054] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-D4-GDI-RhoGDI2-Protein-SDS-Page-NBP1-99054-img0001.jpg "SDS-Page: Recombinant Human D4-GDI/RhoGDI2 Protein [NBP1-99054] - 15% SDS-PAGE (3ug)")
![SDS-Page: Recombinant Human D4-GDI/RhoGDI2 Protein [NBP1-99054] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-D4-GDI-RhoGDI2-Protein-SDS-Page-NBP1-99054-img0002.jpg)
![Western Blot: D4-GDI/RhoGDI2 Overexpression Lysate (Adult Normal) [NBL1-07674] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for D4-GDI/RhoGDI2.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07674-img0002.jpg "Western Blot: D4-GDI/RhoGDI2 Overexpression Lysate (Adult Normal) [NBL1-07674] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for D4-GDI/RhoGDI2.")
![ELISA: Human D4-GDI/RhoGDI2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40360] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40360_human-d4-gdi-rhogdi2-ready-to-use-elisa-kit-colorimetric-211020241528229.png "ELISA: Human D4-GDI/RhoGDI2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40360] - Standard Curve Reference")
![ELISA: Human D4-GDI/RhoGDI2 ELISA Kit (Colorimetric) [NBP3-27229] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27229_human-d4-gdi-rhogdi2-elisa-kit-colorimetric-114202410295919.png "ELISA: Human D4-GDI/RhoGDI2 ELISA Kit (Colorimetric) [NBP3-27229] - Standard Curve Reference")