![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Azide-Free-Western-Blot-NBP2-33248-img0002.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clone | 97A1015 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ARHGDIB |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS - BSA free |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for D4-GDI/RhoGDI2 Antibody (NBP2-33248)Find related products by research area.
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D4-GDI (GDP dissociation inhibitor, RhoGD12) The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M... Read full blog post. |
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| Gene Symbol | ARHGDIB |
![Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33248-img0003.jpg "Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody. Image from the standard format of this antibody.")
![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Azide-Free-Western-Blot-NBP2-33248-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected. Image from the standard format of this antibody.")
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