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UBE4B Antibody

Images

 
Western Blot: UBE4B Antibody [NBP1-84943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-84943] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: UBE4B Antibody [NBP1-84943] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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UBE4B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE4B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
UBE4B Protein (NBP1-84943PEP)
Publications
Read Publication using NBP1-84943.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22864569)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBE4B Antibody

  • E4
  • homologous to yeast UFD2
  • Homozygously deleted in neuroblastoma 1
  • homozygously deleted in neuroblastoma-1
  • KIAA0684HDNB1
  • UBE4B
  • ubiquitin conjugation factor E4 B
  • Ubiquitin fusion degradation protein 2
  • ubiquitination factor E4B (homologous to yeast UFD2)
  • ubiquitination factor E4B (UFD2 homolog, yeast)
  • ubiquitin-fusion degradation protein 2
  • UBOX3
  • UFD2
  • UFD2a
  • UFD2UFD2A

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBE4B Antibody (NBP1-84943)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE4B